Studying Phospholipids Present in Coconut Oil by Different Methods of Analysis

International Journal of Applied Chemistry

© 2024 by SSRG - IJAC Journal

Volume 11 Issue 3

Year of Publication : 2024

Authors : Pritha Nag

10.14445/23939133/IJAC-V11I3P101

How to Cite?

Pritha Nag, "Studying Phospholipids Present in Coconut Oil by Different Methods of Analysis," SSRG International Journal of Applied Chemistry, vol. 11,  no. 3, pp. 1-14, 2024. Crossref, https://doi.org/10.14445/23939133/IJAC-V11I3P101

Abstract:

Over an extended period, phospholipids within a container of manufactured and processed coconut oil begin to settle and separate from the coconut oil, resulting in impurities within the oil. However, as phospholipids provide nutrients and are integral to coconut oil, removing them entirely is inefficient. Instead, understanding the quantity of phospholipids present in coconut oil can help to understand how much to remove without stripping the oil of its nutrients. This research paper delves into three separate methods of analysing the presence and quantity of phospholipids: Thin Layer Chromatography (TLC), High Performance Thing Layer Chromatography (HPTLC) and UV Spectrophotometer. The importance of this study relates to furthering the understanding of phospholipids in coconut oil, and by analysing three of the most popular analytical techniques, the pros and cons can be weighed and used accordingly. In this paper, TLC and HPTLC undertake a qualitative approach as they confirm the presence and quantity of phospholipids through observation and not measurement. UV spectrophotometer undertakes a quantitative approach as it confirms the presence and quantity through measurement. The research in this paper focuses on the workings of each approach, the challenges faced by each method, as well as when each method can prove to be most helpful. Thus, through rigorous investigation and trial and error, this work aims to understand how to quantity and confirm phospholipids. It serves as a foundation for creating purer coconut oil that retains all its essential elements.

Keywords:

Chromatography, Fatty acids, HPTLC, TLC, UV Spectrophotometer.

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