Genetic Diversity among Grapevine (Vitis Vinifera L.) Cultivars of Tartous Province (Syria) using Microsatellite Markers

International Journal of Agriculture & Environmental Science

© 2018 by SSRG - IJAES Journal

Volume 5 Issue 6

Year of Publication : 2018

Authors : Rabab Dauob, Georges Makhoul, Hafez Mahfoud

10.14445/23942568/IJAES-V5I6P109

How to Cite?

Rabab Dauob, Georges Makhoul, Hafez Mahfoud, "Genetic Diversity among Grapevine (Vitis Vinifera L.) Cultivars of Tartous Province (Syria) using Microsatellite Markers," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 6, pp. 54-8, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I6P109

Abstract:

Genetic variation among 32 grapevine (Vitis vinifera L.) accessions belongs to 23 cultivars from Tartous province were analysed using 10 SSR primers (Simple Sequence Repeat). The ten SSR loci generated (44) alleles, of which (32) were polymorphic (72.72%). The number of SSR markers per primer pair ranged from 3 (VVMD8 and SCU10VV) to 7 (VVS5) with an average of 4.4 alleles/primers. Two loci (VVS1 and VVMD8) gave 100% polymorphic alleles. Cluster analysis separated the investigated cultivars in six major groups with a genetic variance of 0.29. High level of genetic diversity obtained in the collection may be attributed due to continuous seed propagation by birds, natural hybridization between local and introduced cultivars and human selection. Levels of polymorphism detected within the studied accessions proved to be sufficiently genetically diverse and therefore these accessions would be useful in grapevine breeding programs.

Keywords:

Grapevine, Genetic diversity, SSR technique, cluster analysis

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