Antimicrobial Activity of Some Crude Marine Mollusca Extracts Against Some Human Pathogenic Bacteria

International Journal of Agriculture & Environmental Science
© 2020 by SSRG - IJAES Journal
Volume 7 Issue 1
Year of Publication : 2020
Authors : Fayez Sake, Omaima Nasser, Ali Sabha
pdf
How to Cite?

Fayez Sake, Omaima Nasser, Ali Sabha, "Antimicrobial Activity of Some Crude Marine Mollusca Extracts Against Some Human Pathogenic Bacteria," SSRG International Journal of Agriculture & Environmental Science, vol. 7,  no. 1, pp. 6-12, 2020. Crossref, https://doi.org/10.14445/23942568/IJAES-V7I1P102

Abstract:

This study evaluated the antimicrobial of methanol, ethanol and acetone tissue extracts of two molluscs, Pinctada radiate (P. radiata) and Brachidonta
variabilis (B .variabilis). Agar diffusion and broth dilution assays were used to test for antimicrobial activity against five nosocomial bacteria including, Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli . Extracts of both molluscs showed significant activity against all the bacteria strains tested. The best antibacterial activity was recorded by methanol extracts of B. variabilis towards Pseudomonas aeruginosa.
Ethanol extracts of B. variabilis had greater overall activity against all test microbes. Ethanol extracts had higher antimicrobial activity index than their methanol and acetone counterparts. The findings of this work indicate that the tissue extracts of B. variabilis and P. radiataare promising sources of antimicrobial agents that can be utilized for pharmaceutical and nutraceutical purposes.

Keywords:

Molluscs, Antimicrobial Activity, natural products, Pinctadaradiata, Brachidonta variabilis, Nosocomial Pathogens.

References:

[1] Haszprunar, G. (2001). "Mollusca(Molluscs)". Encyclopedia of Life Sciences. John Wiley & Sons, Ltd..doi:10.1038/npg.els.0001598
[2] Iwu, M. M., Duncan, A. R., &Okunji, C. O. (1999). New antimicrobials of plant origin. In J. Janick (Ed.), Perspectives on new crops and new uses (Vol. 9, pp. 51–56). Alexandria, VA: ASHS Press.
[3] Franklin, T. J., & Snow, G. A., (2013). Biochemistry of antimicrobial action, 11. New York, NY: Springer.
[4] Prescott, L., Harley, J., & Klein, D. A. (2002). Microbiology (5th ed.). London: McGraw-Hill.
[5] Sneader, W. (2005). Drug discovery: A history. Chichester: Wiley.10.1002/0470015535
[6] Skropeta, D. (2008). Deep-sea natural products. Natural Product Reports,25, 1131–1166.10.1039/b808743a
[7] Thakur, N. L., Thakur, A. N., & Müller, W. E. G. (2005). Marine natural products in drug discovery. Natural Product Radiance,4, 471–477.
[8] Jimeno, J., Faircloth, G., Sousa-Faro, J. F., Scheuer, P., & Rinehart, K. (2004). New marine derived anticancer therapeutics—A journey from the sea to clinical trials. Marine Drugs,2, 14–29.10.3390/md201014
[9] Newman, D. J., &Cragg, G. M. (2004). Marine natural products and related compounds in clinical and advanced preclinical trials. Journal of Natural Products,67, 1216–1238.10.1021/np040031y
[10] Benkendorff, K. (2010). Molluscan biological and chemical diversity: Secondary metabolites and medicinal resources produced by marine molluscs. Biological Reviews,85, 757–775.
[11] Faulkner, D. J. (2001). Marine natural products. Natural Product Reports,18(1), 1–49.10.1039/b006897g
[12] Kamiya, H., Muramoto, K., Goto, R., Sakai, M., Endo, Y., & Yamazaki, M. (1989). Purification andcharacterization of an antibacterial and antineoplastic protein secretion of a sea hare, Aplysia Juliana. Toxicon, 27(12), 1269-1277.
[13] PremAnand, T., Rajaganapathi, J., & Edward, J. P. (1997). Antibacterial activity of marine molluscs fromPortonovo region. Indian journal of marine sciences, 26(2), 206-208.
[14] Grabley, S., &Thiericke, R. (1999). Bioactive agents from natural sources: trends in discovery andapplication Thermal Biosensors, Bioactivity, Bioaffinitty (pp. 101-154): Springer.
[15] Simmons, T. L., Andrianasolo, E., McPhail, K., Flatt, P., &Gerwick, W. H. (2005). Marine natural productsas anticancer drugs. Molecular Cancer Therapeutics, 4(2), 333-342.
[16] Aneiros, A., &Garateix, A. (2004). Bioactive peptides from marine sources: pharmacological properties andisolation procedures. Journal of Chromatography B, 803(1), 41-53.
[17] Addae-Mensah, I., & Achenbach, H. (1985). Terpenoids and flavonoids of Brideliaferruginea. Phytochemistry,24, 1817–1819.10.1016/S0031-9422(00)82558-3.
[18] Dadson, B. A., &Minta, A. (1976). Isolation, identification, and synthesis of rubesamide, a new naturally occurring cyclopropanecarboxamide from fagararubescens. Journal of the Chemical Society, Perkin Transactions,1, 146–147.10.1039/p19760000146
[19] Ekuadzi, E., Dickson, R. A., Fleischer, T. C., Amponsah, I. K., Pistorius, D., &Oberer, L. (2014). Chemical constituents from Gouanialongipetala and Glyphaeabrevis. Natural Product Research,28, 1210–1213.10.1080/14786419.2014.921685
[20] Narayanasamy, V. (1995). Pharmacopeia of hospital of Indian medicine, Part-II, Siddha. Tamilnadu SiddhaMedical Board Publication, Chennai, India, 203.
[21] Becerro, M. A., Lopez, N. I., Turon, X., &Uriz, M. J. (1994). Antimicrobial activity and surface bacterialfilm in marine sponges. Journal of Experimental Marine Biology and Ecology, 179(2), 195-205.
[22] Wright, A. E. (1998). Isolation of marine natural products. Natural Products Isolation, 365-408.
[23] Kelman, D., Kashman, Y., Rosenberg, E., Ilan, M., Ifrach, I., &Loya, Y. (2001). Antimicrobial activity ofthe reef sponge Amphimedonviridis from the Red Sea: evidence for selective toxicity. Aquatic MicrobialEcology, 24(1), 9-16.
[24] Concepcion, G. P., Caraan, G. B., Lazaro, J. E., &Camua, A. R. (1994). Antibacterial and antifungal activitydemonstrated in some Philippine sponges and tunicates. Journal of Microbial Infection and Disease, 24, 6-19. 9
[25] Ghosh, S., Subudhi, E., &Nayak, S. (2008). Antimicrobial assay of Stevia rebaudianabertoni leaf extracts against 10 pathogens. International Journal of Integrative Biology,2(1), 1–5.
[26] Sathyan, N., Chaithanya, E. R., Anil Kumar, P. R., Sruthy, K. S., & Philip, R. (2014). Comparison of the antimicrobial potential of the crude peptides from various groups of marine molluscs. International Journal of Research in Marine Sciences,3, 16–22.
[27] Anand, T. P., & Edward, J. K. P. (2002). Antimicrobial activity in the tissue extracts of five species of cowries cypraea spp. (Mollusca: gastropoda) and an ascidian didemnumpsammathodes (Tunicata: didemnidae). Indian Journal of Marine Sciences,31, 239–242.
[28] Karthikeyan, S. C., Velmurugan, S., Donio, M. B. S., Michaelbabu, M., &Citarasu, T. (2014). Studies on the antimicrobial potential and structural characterization of fatty acids extracted from Sydney rock oyster Saccostreaglomerata. Annals of Clinical Microbiology and Antimicrobials,13(1), 1–
11.
[29] Kiran, N., Siddiqui, G., Khan, A. N., Ibrar, K., &Tushar, P. (2014). Extraction and screening of bioactive compounds with antimicrobial properties from selected species of mollusk and crustacean. Journal of Clinical & Cellular Immunology,5, 1–5.