Kinds of Co-Cultivation In Soybean Plantlets Support Regeneration And Acclimatization (New Kind Tissue Culture Release)

International Journal of Agriculture & Environmental Science

© 2020 by SSRG - IJAES Journal

Volume 7 Issue 6

Year of Publication : 2020

Authors : Istiyono Kirno Prasetyo, M Adri Budi S

10.14445/23942568/IJAES-V7I6P106

How to Cite?

Istiyono Kirno Prasetyo, M Adri Budi S, "Kinds of Co-Cultivation In Soybean Plantlets Support Regeneration And Acclimatization (New Kind Tissue Culture Release)," SSRG International Journal of Agriculture & Environmental Science, vol. 7,  no. 6, pp. 55-58, 2020. Crossref, https://doi.org/10.14445/23942568/IJAES-V7I6P106

Abstract:

Plant breeding activities with the insertion of genes can also be done, this technique is easier done on tissue culture technique was compared with similar techniques in normal plants. This technique is made possible on the degree of success of the insertion of the gene are more successful than if insertion is done in mature plants, as well as the insertion of resistance genes against fungal pathogens on soybean plants. Genes glucanase and chitinase from Trichoderma harzianum, a fungal parasite that produces enzymes chitinolytic and glucanolytic. Therefore, exploration and trial are needed to be able to infect Agrobacterium easily into soybean plants. So that a healthy and strong tissue culture plantlet is obtained to be continued into the next stage. Successful insertion explain will be able to regenerate their cells to grow into planlet. Furthermore, planet with media nutrient intake will develop into plants in tissue culture bottles, where good plants will produce fresh primordial and radicular growth. After about twenty-eight days, the micro plant is ready to be moved in the grower medium. The Methods of this research are Callus as experimental material taken from three sources and grouped according to these sources: (1) The callus on the results of previous regeneration; (2) callus from hypocotyl; (3) Callus of Cotyledon. Each of the callus sources used as a component of the first factor (Kl) Regeneration of Calli Calli material; (Kh) Material callus from hypocotyl and (Kt) of Cotyledon Calli material. The co-cultivation method used is a modification in 3 ways after co-cultivation modification. The third way is used as the basis for modification of the design of the second-factor components: (Ks) Filter Paper Method After cultivation; (Md) Solid Media Method After cultivation; (Pc) Washing Method After Cultivation. From the research, observations and discussions can be concluded as follows: (1) There was an interaction between the modified method with source material Soybean Callus; (2) Modification of a very good method of this study was Laundering and Paper Filter; (3) Callus excellent source of this research is of regeneration callus and Cotyledon

Keywords:

Plasmid, Regenerate, Tissue Culture, Callus, Acclimatization

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