The Bioactivity of bacillus sp. Marine Bacteria Isolated From Marine Sediments of Lattakia City Towards Pathogenic Bacteria

International Journal of Agriculture & Environmental Science

© 2021 by SSRG - IJAES Journal

Volume 8 Issue 2

Year of Publication : 2021

Authors : Rami HAMMOD, Ahmed QARA ALI, Badr Al ALI

10.14445/23942568/IJAES-V8I2P105

How to Cite?

Rami HAMMOD, Ahmed QARA ALI, Badr Al ALI, "The Bioactivity of bacillus sp. Marine Bacteria Isolated From Marine Sediments of Lattakia City Towards Pathogenic Bacteria," SSRG International Journal of Agriculture & Environmental Science, vol. 8,  no. 2, pp. 30-34, 2021. Crossref, https://doi.org/10.14445/23942568/IJAES-V8I2P105

Abstract:

The study included the selection of local marine bacteria isolates that were isolated from the sediments of the coastal waters of Lattakia, and their identity was determined using a number of agricultural, morphological, and biochemical tests as being species belonging to the genus Bacillus. The inhibitory activity of different concentrations of the organic extracts of these bacterial isolates against some pathogenic bacteria (Streptococcus pneumonia and Staphylococcus aureus) and Gram-positive bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia) were studied using the gram-negative diffusion method. The inhibitory effect of this organic extract was then compared to that of some commercial antibiotics. The results showed that the biological activity of the organic extract of isolates of the genus Bacillus sp (B. subtilis, B. polymyxa, B. cereus, B. circulans) showed that the largest inhibition halo diameter of B. subtilis extract towards S. aureus was 26 mm, and it was the lowest halo diameter for bacteria. P. aeruginosa was 13mm. As for B. polymyxa extract, the highest inhibition halo diameter was 27 mm against S. pneumoniae and 25 mm against S. aureus, and the lowest diameter of inhibition was recorded as 13 mm against P. aeruginosa. In relation to the extracts of other species of the genus Bacillus sp (B. cereus, B. circulans), the inhibitory activity of their extracts was higher against Gram-positive bacteria than against Gram-negative bacteria. These bacterial extracts of Bacillus sp were more effective than various commercial antibiotics used.

Keywords:

Bacillus, Antibacterial activity, pathogenic bacteria.

References:

[1] ANSARUZZAMAN, M.; NAIR, G.B. and ENDRZ, H.P. Antibacterial Substance Produced Pseudomonas aeruginosa with High In-Vitro Antimicrobial Activity against Vibrio cholera O1 and O139. Scientific Session, 1 February. 2010. http://www.sciencedirect.com/.
[2] APPELBAUM, P.C. Reduced Glycopeptide Susceptibility in Methicillin-Resistant Staphylococcus aureus (MRSA). International Journal of Antimicrobial Agents, 30(2007) 398-408.
[3] BUCHANAN, R. E. and GIBBONS, N. E. Bergey’s Manual of Determinative Bacteriology. Eight Edition, William & Wilkins, Baltimore, USA., (1974) 1-573.
[4] CUNHA, M.A.; ASSUNCAO, G.L.M.; MEDEIROS, I.M., and FREITAS, M.R. Antibiotic Resistance Patterns of Urinary Tract Infections in a Northeastern Brazilian Capital. Rev. Inst. Med. Trop. Sao Paulo, 58(2)(2016) 1-4.
[5] DASH, S.K.; CHAKRABORTY, S.P.; MANDAL, D. and ROY, S. Isolation and Characterization of Multi-Drug Resistant Uropathogenic Escherichia coli from Urine Sample of Urinary Tract Infected Patients. International Journal of Life Science & Pharma Research, 2(1)(2012) 25-39.
[6] GAD, A.; BELTAGY, E.; ABDUL-RAOUF, U.; EL-SHENAWY, M. and ABOUELKHEIR, S. Screening of Marine Fatty Acids Producing Bacteria with Antimicrobial Capabilities. Chemistry of Advanced Materials,1(2)(2016) 41-54.
[7] GARRITY, G.M.; BELL, J.A. and LIBURRN, T.G. Taxonomic Outline of the Prokaryotes Bergey,s Manual of Systematic Bacteriology. 2nd Edition, Springer, New York Berlin
Heidelberg, (2004) 401.
[8] IBTISSAM, C.; HASSANE, R.; JOSÉ, M-L.; FRANCISCO, D.S.J.F.; ANTONIO, G.V.J.; HASSAN, B. and MOHAMED, K. Screening of Antibacterial Activity in Marine Green and Brown Macroalgae from the Coast of Morocco. African Journal of
Biotechnology, 8(7)(2009) 1258-1262.
[9] KABORE, W.A.D.; BAGRE, T.S.; KONATE, A.; TRAORE, R.G.; BAKO, E.; KONSEM, T.; BOISRAME, S.; TRAORE, A.S.; BARRO, N. and SANGARE, L .Characteristics of Streptococcus and Staphylococcus strains Isolated from Acute Cellulitis of Dental Origin in Ouagadougou, Burkina Faso. African Journal of Microbiology Research,10(9)(2016) 292-300.
[10] KATZ, E. and DEMAIN, A.L. The Peptide Antibiotics of Bacillus: Chemistry, Biogenesis, and Possible Functions. Bacteriology Reviews, 41(2)(1977) 449-474.
[11] KAWAMOTO, J.; KURIHARA, T.; YAMAMOTO, K.; NAGAYASU, M.; TANI, Y.; MIHARA, H.; HOSOKAWA, M.; BABA, T.; SATO, B. and ESAKI, N. Eicosapentaenoic Acid Plays a Beneficial Role in Membrane Organization and Cell Division of a Cold-Adapted Bacterium, Shewanella livingstonensis Ac10. Journal of Bacteriology, 19(2)(2009) 652-640.
[12] NCCLS. Performance Standards for Antimicrobial Susceptibility Testing. Fourteenth informational supplement. 24(1)(2004).
[13] OBEROI, S.S.; DHINGRA, C.; SHARMA, G. and SARDANA, D. Antibiotics in Dental Practice: How Justified Are We?. 65(2015) 4-10.
[14] ONBASLI, D. and ASLIM, B. Determination of Antimicrobial Activity and Production of Some Metabolites by Pseudomonas aeruginosa B1 and B2 in Sugar Beet Molasses. African Journal of Biotechnology. 7(24)(2008) 4614-4619.
[15] RICE, L.B. Antimicrobial Resistance in Gram-Positive Bacteria. The American Journal of Medicine,119(6)(2006) 11-19.
[16] SAHA, S.; THAVASI, R. and JAYALAKSHMI, S. Phenazine Pigments from Pseudomonas aeruginosa and Their Application as Antibacterial Agent and Food Colourants. Research Journal of Microbiology,3(3)(2008) 122-128.
[17] SAOSOONG, K.; WONGPHATHANAKUL W.; POASIRI, C. and RUANGVIRIYACHAI, C. Isolation and Analysis of Antibacterial Substance Produced from P. aeruginosa TISTR 781. KKU Science Journal, 37(2)(2009) 163-172.
[18] SCHOENTAL, R. The Nature of the Antibacterial Agents Present in Pseudomonas pyocyaneus Culture. The British Journal of Experimental Pathology, 22(3)(1941) 137-147.
[19] THOMSON, J.M. and BONOMO, R.A. The Threat of Antibiotic Resistance in Gram Negative Pathogenic Bacteria: β-lactams in peril!. Current Opinion in Microbiology, 8(5)(2005) 518-524.
[20] Todar, K. Todar's on line Textbook of Bacteriology University of Wisconsin-Madison, Department of Bacteriology, (2003).
[21] UZAIR, B.; AHMED, N.; KOUSAR, F. and EDWARDS, D.H. Isolation and Characterization of Pseudomonas Strain That Inhibit Growth of Indigenous and Clinical Isolate. The Internet Journal of Microbiology, 2(2)(2006) 16(2011). http://www.sciencedirect.com/.
[22] WHO. Antimicrobial Resistance: Global Report on Surveillance. World Health Organization, (2014) 1-232.
[23] WHO. The Evolving Threat of Antimicrobial Resistance Options for Action. World Health Organization. (2012) 1-125 (2012).
[24] WHO. The WHO Policy Package to Combat Antimicrobial Resistance. Geneva. World Health Organization, (2011).
[25] YANG, R.-Y.; LI, C.-Y.; LIN, Y-C.; PENG, G.-T.; SHE, Z.-G. and ZHOU, S.-N. Lactones from a Brown Alga Endophytic Fungus from the South China Sea and Their Antimicrobial Activities. Bioorganic & Medicinal Chemistry Letters, 16(36)(2006) 4205-4208.
[26] ZHENG, L.; HAN, X.; CHEN, H.; LIN, W. and YAN X. Marine Bacteria Associated with Marine Macroorganisms: the Potential Antimicrobial Resources. Annals of Microbiology, 55(2)(2005) 119-124.
[27] DASH, S.K.; CHAKRABORTY, S.P.; MANDAL, D.; ROY, S. Isolation and Characterization of Multi Drug Resistant Uropathogenic Escherichia coli from Urine Sample of Urinary Tract Infected Patients. International Journal of Life Science & Pharma Research, 2(1)(2012) 25-39.