The Efficacy of Crinum Zeylanicum L. Leaf Extract Analysis by GC-MS in Inhibiting Mycelial Growth of Sclerotinia Sclerotiorum Isolates on Common Bean (Phaseolus Vulgaris L.)

International Journal of Agriculture & Environmental Science

© 2024 by SSRG - IJAES Journal

Volume 11 Issue 5

Year of Publication : 2024

Authors : Claire Stéphane Tsogo, Patrice Zemko Ngatsi, Nobert William Tueguem Kuate, Marie Elvia Akong, Thierry Songwe Atindo, Hubert Boli, Nadège Wandji Tchasep, Bekolo Ndongo

10.14445/23942568/IJAES-V11I5P101

How to Cite?

Claire Stéphane Tsogo, Patrice Zemko Ngatsi, Nobert William Tueguem Kuate, Marie Elvia Akong, Thierry Songwe Atindo, Hubert Boli, Nadège Wandji Tchasep, Bekolo Ndongo, "The Efficacy of Crinum Zeylanicum L. Leaf Extract Analysis by GC-MS in Inhibiting Mycelial Growth of Sclerotinia Sclerotiorum Isolates on Common Bean (Phaseolus Vulgaris L.)," SSRG International Journal of Agriculture & Environmental Science, vol. 11,  no. 5, pp. 1-11, 2024. Crossref, https://doi.org/10.14445/23942568/IJAES-V11I5P101

Abstract:

White mold caused by Sclerotinia sclerotiorum is a disease that attacks more than 400 plant species. Controlling it in the field requires the use of chemical fungicides, which are toxic and polluting. This work aimed to estimate the effectiveness of bioactive compounds present in Crinum zeylanicum leaf extracts against Sclerotinia sclerotiorum isolates. Thus, the aqueous, acetone and methanol leaf extracts of C. zeylanicum were obtained, analyzed by GC-MS and tested at different concentrations on mycelial growth and spores germination of S. sclerotiorum isolates. MIC50 and MIC90 (minimum inhibitory concentrations) were determined. Results showed that plant extracts are rich in phytochemical compounds, with 39, 32, and 10 being methanol, acetone, and aqueous extracts, respectively. Many of these compounds were detected to have biocidal activity. Total inhibition (100%) was obtained at 120 μg/mL with aqueous extract and 60 μg/mL with methanol and acetone extracts. No MIC50 could be determined with methanol extracts in both isolates. The lowest inhibitory concentrations were then obtained, with acetone extracts with 1.51 and 0.35 μg/mL for isolates 1 and 2. The highest MIC90 were obtained with the aqueous extract (11.39 and 11.53 μg/mL for isolates 1 and 2). Extracts also led to a total inhibition of spore germination from 60 μg/mL with aqueous extract and from 30 μg/mL with acetone and methanol extracts. These results sufficiently demonstrate the richness in active molecules of C. zeylanicum leaf extracts and their huge potential in bean white mold control.

Keywords:

Crinum zeylanicum, GC-MS analysis, Plant extract, Sclerotinia sclerotiorum.

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