Tissue Culture and Establishment of Rapid Propagation System of Valeriana jatamansi Jones

International Journal of Agriculture & Environmental Science

© 2017 by SSRG - IJAES Journal

Volume 4 Issue 4

Year of Publication : 2017

Authors : YU Le, LU Hui, JIANG Zong-qing, YANG ZI-xiang

10.14445/23942568/IJAES-V4I4P104

How to Cite?

YU Le, LU Hui, JIANG Zong-qing, YANG ZI-xiang, "Tissue Culture and Establishment of Rapid Propagation System of Valeriana jatamansi Jones," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 4, pp. 22-26, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I4P104

Abstract:

in order to study the rapid and complex system of Valeriana jatamansi Jones (Valeriana wallichii DC.), it provides reference data for the factory cultivation. Tissue cultural and rapid propagation techniques for Valeriana jatamansi Jones were studied through rhizome buds, leaves, stalksas explants. The optimum conditions were selected through the ratio of different hormone concentrations. It turned out that rhizome bud of Valeriana jatamansi Jones was the most suitable explants. Valeriana jatamansi Jones was a perennial herb, its dry rhizome and root can be medicine, currently wild resources were deficient. Tissue cultural and rapid propagation techniques for Valeriana jatamansi Jones were studied through rhizome buds, leaves, stalksas explants. It turned out that rhizome bud of Valeriana jatamansi Jones was the most suitable explants. The suitable callus induction medium was MS medium containing 3.0 mg·L-1 6-BA and0.1 mg·L-1 2, 4-D, the induction rate was 88.7%; the suitable proliferation medium was MS medium containing 3.0 mg·L-1 6-BA and 0.3 mg·L-1 NAA, the proliferation rate was 93.3%;the suitable rooting medium was 1/2MS medium containing 1.5 mg·L-1 NAA, the rooting rate was 100%.

Keywords:

Valeriana jatamansi Jones; Explants; Tissue cultural; Rapid propagation system

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