Dynamic expression of TRPV6, cal bind in D28k and PMCA 1b in the egg during the oviposition cycle in laying hens

International Journal of Agriculture & Environmental Science

© 2017 by SSRG - IJAES Journal

Volume 4 Issue 5

Year of Publication : 2017

Authors : Xiaohu Zhai, Weihua HE, Jiafa HOU, Junhua YANG

10.14445/23942568/IJAES-V4I5P102

How to Cite?

Xiaohu Zhai, Weihua HE, Jiafa HOU, Junhua YANG, "Dynamic expression of TRPV6, cal bind in D28k and PMCA 1b in the egg during the oviposition cycle in laying hens," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 5, pp. 4-12, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I5P102

Abstract:

The aim of this study is to investigate the effect of active Ca2+ transcellular transport on the eggshell calcification. Forty ISA laying hens at the peak stage (220 days old) were assigned to five reproductive stages for sampling. The samples of eggshell gland (ESG) were obtained by the chickens were sacrificed at 0, 2, 4.5, 8 and 16 h after post-oviposition, respectively. Then, quantization’s of mRNA level and protein concentration of TRPV6, CaBP-D28K and PMCA 1b at different stages in the ESG were carried out by real-time PCR and western-blot analysis, respectively. The results are as follows: the expression levels of TRPV6, CaBP-D28K and PMCA 1b mRNA in the ESG were retained very low until the egg movement into the shell gland (0~4.5 h after ovulation), then significantly increased at 16 h during eggshell calcification. In addition, TRPV6, and CaBP-D28K indicated significant statistical difference (P＜0.01), respectively. Furthermore, western blotting showed that the expression of TRPV6 and PMCA 1b reached the maximum at 16 h after ovulation, but the statistical difference was not significant. The change of CaBP-D28K expression was very similar to that of TRPV6, but the concentration was significantly increased at 16 h than that at 0 h after ovulation (P＜0.05). In conclusion, the expression of TRPV6, CaBP-D28K and PMCA 1b in the ESG was regulated by the oviposition cycle, suggesting that active Ca2+ transcellular transport exerted significant effects in calcium delivering in the ESG.

Keywords:

ESG; TRPV6; CaBP-D28K; PMCA 1b; the oviposition cycle

References:

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