Effect of Three Species of Rhizobacteria (PGPR) in Stimulating Systemic Resistance on Tomato Plants against Cucumber Mosaic Virus (CMV)

International Journal of Agriculture & Environmental Science

© 2017 by SSRG - IJAES Journal

Volume 4 Issue 6

Year of Publication : 2017

Authors : Ramez Al Shami, Dr. Imad Ismail, Dr. Yaser Hammad

10.14445/23942568/IJAES-V4I6P103

How to Cite?

Ramez Al Shami, Dr. Imad Ismail, Dr. Yaser Hammad, "Effect of Three Species of Rhizobacteria (PGPR) in Stimulating Systemic Resistance on Tomato Plants against Cucumber Mosaic Virus (CMV)," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 6, pp. 11-16, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I6P103

Abstract:

This experiment aimed to study the effect of three species of plant growth promoting rhizobacteria (Frateuria aurantia, Bacillus megaterium and Azotobacter chroococcum) inoculated to seeds and shoots of tomato plants on Cucumber mosaic virusdisease severity, salicylic acid and peroxidase activity content and their ability to suppress the effect of CMV in a plastic tunnel in Tartus-Syria. The results showed that, the treatment with single bacteria Frateuria aurantia produced significant reduction in disease severity and higher infree salicylic acid and peroxidase activity contents compared with Bacillus megaterium or Azotobacter chroococcumin CMV-infected or healthy controls. Mixed treatments with three bacterial species gave the highest reduction in disease severity and increasedof free salicylic acid and peroxide activity contained in both CMV-infected and healthy tomato plants. Such increase in free salicylic acid and peroxidase activity contents suggested the potential ability of rhizobacteria to stimulate mechanisms of systemic resistance and reduces the effect of CMV infection on tomato plants.

Keywords:

PGPR, CMV, Tomato, Disease Severity, Salicylic Acid, Peroxidase Enzyme.

References:

1. Group agriculture statics (2014). The Directorate of statistics and planning, Ministry of agriculture and agrarian reform, Syrian Arab Republic. In Arabic.
2. G. P. Martelli, & A. Quacquarelli, (1983). "The present status of Tomato and pepper viruses". Acta Horticulturae. (ISHS). 127: 39-64.
3. P. Soleimani, G. Mosahebi, M. K. Habibi, (2011). Identification of some viruses causing mosaic on lettuce and characterization of Lettuce mosaic virus from Tehran Province in Iran. Afr. J. Agric. Res. 6 (13), 3029-3035.
4. A. K. Brunt, M. Carbtree, A. Dallwitz, Gibbs and L. Watson. (1996). Viruses of plants: descriptions and lists from the VIDE database. CAB. International. Printed and bound in the UK at the University press, Cambridge. 1484pp.
5. P. Sutic, D. D. For, R. E. Tosic, (1999). Hand book of plant virus diseases. CRC prees. 553pp.
6. G. N. Agrios, (2005). Plant pathology, 5thed. Elsevier,. 922p.
7. R. Cerkauskas, (2004). Cucumber mosaic virus (CMV). Published by AVRDC- The World Vegetable Center, P.O. Box 42. Shanhua, Taiwan 741, ROC.
8. H. Khalil, (2007). Investigation of viral diseases on tomato plants in Central and coastal area. Journal of Albaath University. Syria, volume 29 number (2), p231-246. In Arabic.
9. H. Kawas, (2006). Studies on tomato viral diseases in southern Syria, and screening cultivars for resistance to infection with viruses. Ninth Arab Congress of Plant Protection, Damascus, Syria. 19-23 November, 2006, Damascus. Syria.
10. T. M. Abdel Ghany, M. M. Alawlaqi, M. A. Al Abboud, (2013). Role of Biofertilizers In Agriculture: A Brief Review. Review Article. Mycopath 11, 2. p 95-101.
11. J. S. Singh, (2013). Plant Growth Promoting Rhizobacteria Potential Microbes for Sustainable Agriculture. (Central) University, Raibarely Road, Lucknow 226025 Uttar Pradesh, India. 2013.pp7.
12. B. S. Saharan, and V. Nehra, 2011. Plant Growth Promoting Rhizobacteria: A Critical Review. Life Sciences and Medicine Research, Volume: LSMR-21.
13. B. Bouizgarne, (2013). Bacteria for Plant Growth Promotion and Disease Management. Springer 454p 40 illus. hardcover.
14. Z. A. Siddiqui, and M. S.Akhtar, (2007). Biocontrol of a chickpea root-rot disease complex with phosphate solubilizing microorganisms. Journal of Plant Pathology. 89:31-42.
15. C. J. Jeffries, (1998). Potato. FAO/IPGRI technical guidelines for the safe movement of germplasm. 19, 62-63.
16. Y. Hammad, and R. Al-Shami, (2017). Identification some species of Plant Growth Promoting Rhizobacteria from some Biofertilizer and Soils. Journal of Albaath University. Syria. Vol 39. P 25. In Arabic.
17. Technical Data. (2011). Pikovskayas Agar M520. HiMedia Laboratories. P2. AshbysMannitol Agar M706. HiMedia Laboratories. P3.
18. P. Lisdiyanti, Y. Yamada, T. Uchimura, K. Komagata, (2003). Identification of Frateuriaaurantia Strains Isolated from Indonesian Sources. Microbiol.Cult. Coll. Vol. 19, No. 2. Dec. 2003. p. 81 -90.
19. J. F. Murphy, M. S. Reddy, Ch.-M. Ryu, J. W. Kloepper and R. LI, (2003). Rhizobacteria- Mediated Growth Promotion of Tomato Leads to Protection Against Cucumber mosaic virus. Phytopathology. 93. p1301-1307.
20. J. Maria Gil and V J ctor Mart J nez-Merino. (2007). Determination of The Free Salicylic Acid Concentration in Aspirin Byforming Fe+3 Complexes. P8.
21. R. Hammerschmidt, E. M. Nuckles, and J. Kuc, (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol. Plant Pathol., 20: 73-82.
22. B. Behera, S. Ghanty, F. Ahmad, S. Santra, S. Banerjee. (2012). UV-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. Analytical &Bioanalytical Techniques. J Anal Bioanal Techniques. Volume 3 . Issue 6.
23. G. Raupach, S. Liu, L. John, F. Murphy, S. Tuzun, and J. W. Klopper, (1996). Induced Systemic Resistance in Cucumber and Tomato Against Ccumber Mosaic Cucumo virus Using Plant Growth Promoting Rhizobacteria (PGPR). Plant Dis. 80:891-894.
24. J. W. Kloepper, C-M. Ryu, S. A. Zhang, (2004). Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94, 1259-1266.
25. K. Jetiyanon, and J. W. Kloepper, (2002). Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control 24. 285-291.
26. KH. A. El-Douggoug, A. A. Megahed, B. A. Othman, S. M. Lashin, M. A. Ibrahim and I. H. Attitalla, (2013). Induction of Salicylic Acid in Cucumber Plants Against Cucumber Mosaic Cucumovirus Using Biotic Inducers. American Journal of Biochemistry and Molecular Biology 3(2): 258-255.
27. G. W. Zehnder, Y. Changbin, J. F. Murphy, E. R. Sikora, and J. W. Klopper, (2000). Induction of Resistance in Tomato Against Cucumber mosaic virus by Plant Growth- Promoting Rhizobacteria. Printed in the Netherlands Biocontrol 45.. p127-137.
28. G. W. Zehnder, J. F. Murphy, E. J. Sikora, and J. W. Kloepper, (2001). Application of rhizobacteria for induced resistance. European Journal .of Plant Pathology 107: P 39-50.
29. S. Sivasakthi; G. Usharani & P. Saranraj, (2015). Biocontrol potentiality of plant growth promoting bacteria (PGPR) Pseudomnas fluorescens and Bacillus subtils: A review. African Journal of Agriculture research. 9(16) .8 pp. 1265-1277.
30. A. M. M. Mahdy, M. A. Hafez, Kh. A. EL-Dougdoug, R. N. Fawzy, and S. M. Shahwan, (2010). Effect Of Two Biotic Inducers on Salicylic Acid Induction in Tomato Infected with Cucumber Mosaic Cucumovirus. 3rd Inter. Conf. Virol., Cairo Univ. Center, Nov. 24-25. Egyptian J. Virol, SP. Issue, 355-372.
31. Kh. A. El-Dougoug, M. F. Ghaly and M. A. Taha, (2012). Biological Control of Cucumber Mosaic Virus by Certain Local Streptomyces Isolates: Inhibitory Effects of Selected Five Egyptian Isolates. International Journal of Virology 8 (2): 151-164.
32. M. Naylor, A. M. Murphy, J. O. Berry, J. P. Carr, (1998). Salicylic acid can induce resistance in plant virus movement. Mol Plant Microbe Interact 11:860-868.
33. A. M. Murphy, A. Gilliland, C. J. York, B. Hyman, J. P. Carr, (2004). High-level expression of alternative oxidase protein sequences enhances the spread of viral vectors in resistant and susceptible plants. J Gen Virol 85:3777-3786.
34. A. M. Murphy, S. Chivasa, D. P. Singh, J. P. Carr, (1999). Salicylic acid-induced rsistance to viruses and other pathogens: a parting of ways? Trends Plant Sci 4:155-160.
35. D. Hondo, S. Hase, Y. Kanayama, N. Yoshikawa, S. Takenaka, H. Takahashi, (2007). The LeATL6 –associated ubiquitin/ proteasome system may contribute to fungal elicitor-activated defense response via the jasmonic acid-dependent signaling pathway in tomato. Mol Plant-Microbe Interact 20:72-81.
36. L. C. Van Loon, (1997). Induced resistance in plants and the role of pathogenesis-related proteins. European Journal Plant Pathology, 103:753-765.
37. L. C. Van Loon, C. M. J. Bakker, and P. A. H. M. Pieterse, (1998). Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology, 36: 453-483.
38. L. C. Van Loon, and E. A. Van Strien, (1999). The families of pathogenesis related proteins and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology, 55:85-97.
39. D. K. Choudhary, A. Prakash, and B. N. Johri, (2007). Induced systemic resistance (ISR) in plants: mechanism of action. Indian Journal of Microbiology. 47 (4): 289-297.
40. X. Nie, (2006). Salicylic acid suppresses Potato virus Y isolate N:O-induced symptoms in tobacco plants. Phytopathology 96:255-263.
41. R. Hammerschmidt, J. P. M´etraux, and L.C. van Loon. (2001). Inducing resistance: a summary of papers presented at the First International Symposium on Induced Resistance to Plant Diseases, Corfu, May 2000. European Journal of Plant Pathology 107: 1-6.
42. J. M. Chittoor, J.E. Leach and F.F. White, (1999). Induction of peroxidase during defense against pathogens. In: Pathogenesis: Related proteins in plants. S.K. Datta, S. Muthukrishnan (eds.). CRC Press, Boca Raton, FL. 291 pp.
43. S. Ebrahim, K. Usha and B. Singh, (2011). Pathogenesis Related (PR) Proteins in Plant Defense Mechanism. Science against microbial pathogens: communicating current research and technological advances.pp12.
44. E. Shahwan, S. M. El-Din. (2010). Inducing systemic resistance against some tomato virus diseases. Dissertation Submitted in Partial Fulfillment of the Requirements for The Degree Of DOCTOR Of PHILOSOPHY In PLANT PATHOLOGY (Viral Diseases). Pp: 256.
45. A. M. El-Borollosy, And M. M. Oraby, (2012). Induced systemic resistance against Cucumber mosaic cucumovirus and promotion of cucumber growth by some plant growth-promoting rhizobacteria. Faculty of Agriculture, Ain Shams University, Annals of Agricultural Science 57(2). p 91-97.
46. P. Lancioni, (2008). Studies on biotic and abiotic elicitors inducing defense responses in tomato. Ph.D. Thesis, Phytopathology Dept., Fac. Agric., University of Bologna, Italy 125pp.