The Effect of Ultraviolet Radiation on Chlorophyll in Chlamydomonas reinhardtii

International Journal of Agriculture & Environmental Science

© 2017 by SSRG - IJAES Journal

Volume 4 Issue 6

Year of Publication : 2017

Authors : Rana Nassour, Abdulkarim Ayash, Issam Mohamad

10.14445/23942568/IJAES-V4I6P105

How to Cite?

Rana Nassour, Abdulkarim Ayash, Issam Mohamad, "The Effect of Ultraviolet Radiation on Chlorophyll in Chlamydomonas reinhardtii," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 6, pp. 22-26, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I6P105

Abstract:

The study was conducted during 2017 in the laboratories of the department of Botany at Tishreen University, Lattakia, Syria. The effect of ultraviolet radiation (UV-C and UV-B) for different periods (0.5, 1, 3, 5, 15, 15, 30, 45, 60 minutes) on the photosynthetic pigments of Chlamydomonas reinhardtii was studied. The results showed that chlorophyll pigments were more sensitive to UV-C compared to UV-B. These reductions were correlated with the increase in the exposure time, reaching the lowest value after 60 minutes exposure (96.36, 90.94% for chlorophyll a and chlorophyll b when measured after 24 hours respectively). The usage of polyethylene films protects the chlorophyll against UV radiation. The protection rate was more evident against UV-C reaching 20.9% after 24 hours in the algae exposed for 30 minutes.

Keywords:

Chlamydomonas reinhardtii, UV-B, UV-C, Chlorophyll, polyethylene films

References:

[1] F. Hollosy, ―Effects of ultraviolet radiation on plant cell, Micron, vol. 33, pp.179- 197, 2002.
[2] H. M. H. Salama, A.A. Al Watban, and A. T. Al-Fughom, ―Effect of ultraviolet radiation on chlorophyll, carotenoid, protein and proline contents of some annual desert plants, Saudi Journal of Biological Sciences, vol. 18, pp.79 – 86, 2011.
[3] S. Shabala, Plant stress physiology- 2nd editon, London, UK, CAB international, 2017.
[4] T. Sreelakshmi and S. H. Raza, ―Effect of UV-A radiation on photosynthetic pigments of selected crop plants, Biolife, vol. 2 (1), pp. 381 – 386, 2014.
[5] L. Xin-shu, F. Zi-hui and H. Pei-min, ―Effects of solar radioation on photosynthesis and pigmentation in the red algae Pyropia yezoensis Udea (Bangiales, Rhodophyta), Indian Journal of Geo- Marine Sciences, vol. 43 (4), pp. 473 – 480, 2014.
[6] N. P. Noyma; T. P. Silva, H. Chiarini-Garcia, A. M. Amado, F. Roland and R. C. K. Melo, ―Potential effects of UV radiation on photosynthetic structures of the bloom-forming cyanobacterium Cylindrospermopsis raciborskii CYRF-01, Frontiers in Microbiology 6, pp. 1-13, 2015.
[7] A. Holzinger, and C. Lütz, ―Algae and UV irradiation: Effects on ultrastructure and related metabolic functions, Micron, vol. 37, pp. 190- 207, 2006.
[8] M. F. Pessoa, ―Harmful effects of UV radiation in Algae and aquatic macrophytes- A review, Emir. J. Food Agric, vol. 24 (6), pp. 510-526, 2012.
[9] E. H. Harris, ―Chlamydomonas as model organism, Annual Review of Plant Physiol. Plant Mol. Biol, vol. 52, pp. 363 – 406, 2001.
[10] E. H. Harris, The Chlamydomonas- source book, 2nd edition, USA, Elsevier Inc, 2009.
[11] R. Bhandari, and P. K. Sharma, ―Effect of UV-B on photosynthesis, membrane lipids and MAAs in marine cyanobacterium Phormidium corium (Agardh) Gomont, Indian Journal of experimental biology, vol. 44, pp. 330 – 335, 2006.
[12] F. Reboredo, and F. J. C. Lidon, ―UV-B radiation effects on terrestrial plants – a perspective, Emir. J. Food Agric., vol. 24 (6), pp. 502 – 509, 2012.
[13] M. Pessarakli, Handbook of photosynthesis, 2nd edition, USA, CRC Press- Taylor & Francis Group, 2005.
[14] L. C. Rai and N. Mallick, ―Algal responses to enhanced ultraviolet-B radiation," PINSA, vol. 64 (2), pp. 125 – 146, 1998.
[15] E. C. Schmidt, B. G. Nunes, M. Maraschin, and Z. L. Bouzon, ―Effect of ultraviolet-B radiation on growth, photosynthetic pigments, and cell biology of Kappaphycus alvarezii (Rhodophyta, Gigartinales) macroalgae brown strain, Photosynthetica, vol. 48 (2), 161 – 172, 2010.
[16] A. Juneja, R. M. Ceballos, and G. S. Murthy, ―Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review, Energies, vol. 6, pp. 4607-4638, 2013.
[17] S. Kataria, A. Jajoo and K. N. Guruprasd, ―Impact of increasing ultraviolet-B (UV-B) radiation on photosynthetic processes, Journal of Photochemistry and Photobiology B: Biology, vol. 137, pp. 55 – 66, 2014.
[18] I. Malpartida, C. G. Jerez, M. M. Morales, P. Nascimento, I. Freire, J. Ezequiel, R. M. Rico, E. Peralta, J. R. Malapascua, Y. Florez, J. Masojidek, R. Abdala, F. L. Figueroa, and E. Navarro, ―Synergistic effect of UV radiation and nutrient limitation on Chlorella fusca (Chlorophyta) cultures grown in outdoor cylindrical photobioreactors, Aquatic Biology, vol. 22, pp. 141–158, 2014.
[19] K. Tilbrook, M. Dubois, C. D. Crocco, R. Yin, R. Chappuis, G. Allorent, E. Schmid-Siegert, M. Goldschmidt-Clermont, and R. Ulm, ―UV-B perception and acclimation in Chlamydomonas reinhardtii, Plant cell, vol. 28 (4), pp. 966 – 983, 2016.
[20] H. K. Lichtenthaler, ―Chlorophylls and carotenoids: pigments of photosynthetic biomembranes, Methods in enzymology, vol. 148, pp. 350 – 382 , 1987.
[21] R. Sharma, and V. K. Sharma, ―Effect of ultraviolet-B radiation on growth and pigments of Chlorella vulgaris, Journal of Indian botanical Society, vol. 94 (1 & 2), pp. 81 – 88, 2015.
[22] D. Doneva, J. Ivanova and L. Kabaivanova, ―Physiological and biochemical changes in algal cultures of Chlorella vulgaris and Synechocystis salina (mesophilic and antarctic isolates) occuring after treatment with UV-B radiation, Ecological Engineering and Environment Protection, vol. 8, pp. 73 – 82, 2017.
[23] K. Ganapathy, K. Chidambaram, R. Janarthanan, and R. Ramasamy, ―Effect of UV-B radiation on growth, photosynthetic activity and metabolic activities of Chlorella vulgaris, Research & Reviews: Journal of Microbiology and Biotechnology, vol. 6 (2), pp. 53 – 60, 2017.
[24] Y. Gao, Y. Cui, W. Xiong, X. Li, and Q. Wu, ―Effect of UV-C on Algal Evolution and Differences in Growth Rate, Pigmentation and Photosynthesis Between Prokaryotic and Eukaryotic Algae, Photochemistry and Photobiology, vol. 85, pp. 774–782, 2009.
[25] F. Borderie, L. Alaoui-Sehmer, F. Bousta, and B. Alaoui-Sosse, ―Cellular and molecular damage caused by high UV-C irradiation of the cave-harvested green alga Chlorella minutissima: Implications for cave management, International Biodeterioration & Biodegradation, vol. 93, pp. 118 – 130, 2014.
[26] J. Jiang, ―Study on Decreasing Effect of Ascorbic Acid on Algae Killing Ability of UV. Asia-Pacific Energy Equipment Engineering Research Conference (AP3ER 2015), pp. 206- 209, 2015.
[27] C. A. Marwood, and B. M. Greenberg, ―Effect of supplementary UVB radiation on chlorophyll synthesis and accumulation of photosystems during Chloroplast Development in Spirodela oligorrhiza, Photochemistry and Photobiology, vol. 64 (4), pp. 664 – 670, 1996.
[28] S. B. Agrawal, ―Effects of supplemental U.V.-B radiation on photosynthetic pigment, protein and glutathione contents in green algae, Environmental and Experimental Botany, vol. 32 (2), pp. 137 – 143, 1992.
[29] S. H. Sarghein, J. Carapetian, and J. Khara, ―Effects of UV radiation on photosynthetic pigments and UV absorbing compounds in Capsicum longum L., International journal of Botany, vol. 4 (4), pp. 486 – 490, 2008.
[30] S. H. Sarghein, J. Carapetian, and J. Khara, ―The effects of UV radiation on some structural and ultrastructural parameters in pepper (Capsicum longum A.DC.), Turkish journal of Biology, vol. 35, pp. 69 – 77, 2011.
[31] L. L. Eggink, H. Park, and J. K. Hoober, ―The role of chlorophyll b in photosynthesis: Hypreviewothesis, BMC Plant Biology, vol. 1:2, 2001.
[32] B. Grimm, R. J. Porra, W. Rüdiger, and H. Scheer, Chlorophylls and Bacteriochlorophylls Biochemistry, Netherlands, Springer, 2006.
[33] S. Bujaldon, N. Kodama, F. Rappaport, R. Subramanyam, C. de Vitry, Y. Takahashi and F. A. Wollman, ―Functional Accumulation of Antenna Proteins in Chlorophyll b-Less Mutants of Chlamydomonas reinhardtii, Molecular Plant, vol. 10, pp. 115 – 130, 2017.