Isolation and identification of indigenous hydrocarbon tolerant fungi from soil contaminated with Biodiesel in Benin City, Nigeria

International Journal of Agriculture & Environmental Science

© 2017 by SSRG - IJAES Journal

Volume 4 Issue 6

Year of Publication : 2017

Authors : Igiebor F.A., Osarumwense J.O., Obinyan, B.O., Okoye, P.C

10.14445/23942568/IJAES-V4I6P109

How to Cite?

Igiebor F.A., Osarumwense J.O., Obinyan, B.O., Okoye, P.C, "Isolation and identification of indigenous hydrocarbon tolerant fungi from soil contaminated with Biodiesel in Benin City, Nigeria ," SSRG International Journal of Agriculture & Environmental Science, vol. 4,  no. 6, pp. 46-49, 2017. Crossref, https://doi.org/10.14445/23942568/IJAES-V4I6P109

Abstract:

Environmental pollution by hydrocarbons is a serious problem all around the world. Scientists have identified various microorganisms that are effective degraders of these hydrocarbons in natural environments. This study was aimed at isolating and characterizing hydrocarbon degrading fungi from soil contaminated with biodiesel effluent. Five types of indigenous fungi were isolated and identified from the contaminated soil: they were Aspergillus flavus, Aspergillus niger, Aspergillus carmari, Mucor spp., Penicillium notatum. Their biodegradability capability was measured by UV spectroscopy at wavelength of 600nm for 14 days under optimal temperatures at 280C. The isolate with best degradation ability was Aspergillus carmari which has the potentials to be used as hydrocarbon degrading fungus in remediating biodiesel effluent contaminated site.

Keywords:

bioremediation, Biodiesel, fungi, effluent, hydrocarbon

References:

1) Aksoy, H. and Becerik,Y. (1990). Utilization prospects of Turkish raisin seed oil as an alternative engine fuel. Fuel 69: 600–603.
2) Fernández-Álvarez P, Vila J, Garrido-Fernández JM, Grifoll M, Lema JM (2006). Trials of bioremediation on a beach affected by the heavy oil spill of the Prestige. J. Hazard. Mater., 137: 1523–1531.
3) Fernández-Álvarez P, Vila J, Garrido-Fernández JM, Grifoll M, Lema JM (2007). Evaluation of biodiesel as bioremediation agent for the treatment of the shore affected by the heavy oil spill of the Prestige. J. Hazard. Mater. 147(3): 914-922.
4) Kanu, I. and Achi, O. K. (2011). Industrial Effluents and Their impact on Water Quality of Receiving River in Nigeria. Journal of applied technology in Environmental al Sanitation 1(1): 75–86.
5) Lapinskiené A, Martinkus P, Rebzdaite V (2006). Eco-toxicological studies of diesel and biodiesel fuels in aerated soil. Environ. Poll. 142: 432-437.
6) Makareviciene V, Janulis P (2003). Environmental effect of rapeseed oil ethyl ester. Renewable Energy 28: 2395–2403.
7) Mariano, A. P., Tomasella, R. C., Oliveira, L. M., Contiero, J. and Angelis, D. F. (2008).Biodegradability of diesel and biodiesel blends. African Journal of Biotechnology 7 (9): 1323-1328.
8) Obbard JP, NG, KL, XU, R (2004). Bioremediation of petroleum contaminated beach sediments: use of crude palm oil and fatty acids to enhance indigenous biodegradation. Water, Air Soil Pollut. 157: 149- 161.
9) Pasqualino JC, Montané D, Salvadó J (2006). Synergic effects of biodiesel in the biodegradability of fossil-derived fuezls. Biomass Bioenergy 30: 874-879.
10) Richa, S., Chandra, S. and Singh, A. (2013). Isolation of microorganism from soil contaminated with degraded paper in Jharna village. Journal of Soil Science and Environmental Management 4(2): 23-27.
11) Saroj, A. and Keerti, D. (2013). Isolation and characterization of hydrocarbon degrading microorganisms from petroleum oil contaminated soil sites. Bulletin of Environmental and Scientific Research 2 (4): 5-10.
12) Taylor LT, Jones DM (2001). Bioremediation of coal tar PAH in soils using biodiesel. Chemosphere 44: 1131-1136.
13) Winkelmann, M., Hunger, N., Hüttl, R. and Wolf, G. (2009). Calorimetric investigations on the degradation of water insoluble hydrocarbons by the bacterium Rhodococcus opacus 1CP. Thermochimica Acta. 482:12–16.
14) Zhang X, Peterson CL, Reece D, Haws R, Moller G (1998). Biodegradability of biodiesel in the aquatic environment. Trans. ASAE. 41: 1423-1430