Azotobacterchroococcum- A Plant Growth Promotingrhizobacteria formulated as biofertilizer in vermicast carrier

International Journal of Agriculture & Environmental Science

© 2018 by SSRG - IJAES Journal

Volume 5 Issue 1

Year of Publication : 2018

Authors : MaheshManjula .K, Maria HeartinaAdlinVaz .A

10.14445/23942568/IJAES-V5I1P104

How to Cite?

MaheshManjula .K, Maria HeartinaAdlinVaz .A, " Azotobacterchroococcum- A Plant Growth Promotingrhizobacteria formulated as biofertilizer in vermicast carrier," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 1, pp. 21-28, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I1P104

Abstract:

Biofertilizers are preparations containing microorganisms efficient strains that help crop plants uptake nutritent and increase soil fertility. Biofertilizer does not pollute the environment and is more beneficial than chemical fertilizers. Plant Growth Promoting Rhizobacteria (PGPR) are free living, soil borne bacteria, which enhance the plant growth through varies mechanisms like synthesis of growth regulating substances like auxins, cytokinin, and gibberellic acid (GA). In addition, it stimulates rhizospheric microbes, protects the plant from phytopathogens, improves nutrient uptake and ultimately boost up Biological Nitrogen Fixation.The PGPR include genera such as Azotobacter, Azospirillum, Pseudomonas, Burkholderia, Bacillus, and some are members of the Enterobacteriaceae. Azotobacter is a free living nitrogen fixingdiazotroph that has several beneficial effects on the crop growth and yield. The main purpose of this research was to study the plant growth promoting efficiency of rhizobacteria (PGPR) – Azotobacter, when mixed with vermicast carrier material. The present study focused on isolation of Azotobacter using Ashby’s media and Jensen’s media which are specific for Azotobacter and Nitrogen fixing bacteria respectively, biochemical identification of Azotobacter, identification of the isolate by 16srDNA sequencing by DNA barcodingtechnique, production of biofertilizer on vermicast carrier and testing the plant growth promoting efficiency of the formulated biofertilizer. Of the several species of Azotobacter, Azotobacterchroococcum happens to be the dominant inhabitant in arable soils capable of fixing nitrogen in culture media. The results confirmed that, the biofertilizerformulated using Azotobacterchroococcum, named as BIOAZOTO- PHS17 can be efficiently used as an alternate for chemical fertilizers.

Keywords:

Azotobacter, Biofertilizer, Vermicompost, Plant Growth Promoting Rhizobacteria(PGPR), Biological Nitrogen fixation

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