Characterization of proteins in Costus pictus D. Don

International Journal of Agriculture & Environmental Science

© 2018 by SSRG - IJAES Journal

Volume 5 Issue 1

Year of Publication : 2018

Authors : Geena Paul, Augustin A

10.14445/23942568/IJAES-V5I1P105

How to Cite?

Geena Paul, Augustin A, " Characterization of proteins in Costus pictus D. Don," SSRG International Journal of Agriculture & Environmental Science, vol. 5,  no. 1, pp. 29-33, 2018. Crossref, https://doi.org/10.14445/23942568/IJAES-V5I1P105

Abstract:

Proteins in Costus pictus was purified and studied using PAGE, SDS- PAGE, Ammonium Sulphate fractionation, DEAE cellulose anion exchange chromatography and sephadex G-100 gel filtration chromatography. Tris buffer of 0.2M at pH 7.2 was found to be suitable buffer for extracting Costus pictus proteins. Ten percent acryl amide gel concentration for resolving and six percent for stacking gel could resolve proteins efficiently through SDS- PAGE and PAGE. Proteins of basic nature are precipitated with ammonium sulphate in the range of 30 to 50 per cent saturation and acidic proteins were precipitated at higher saturation of ammonium sulphate. Higher concentration of salt was required to elute proteins from DEAE column when protein fraction was salted out with 60 and 50-90 percent ammonium sulphate saturation. Molecular sieving was affected by both salt gradient as well as pH due to the presence of both acidic and basic proteins. 70 to 80 percent sequential precipitation gave a protein having same molecular weight as that of insulin. Purification, sequencing and further studies of this fraction have to be conducted for establishing the antidiabetic property and exploiting Costus protein by modern biotechniques.

Keywords:

Costus pictus, Ammonium sulphate precipitation, SDS-PAGE, PAGE, DEAE anion exchange chromatography, Molecular sieving

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