Research Article | Open Access | Download PDF
Volume 13 | Issue 6 | Year 2026 | Article Id. IJCE-V13I6P118 | DOI : https://doi.org/10.14445/23488352/IJCE-V13I6P118Self-Healing and Biological CO₂ Capture in Concrete Using Microencapsulated Nostoc Sphaericum
Rike Ricardo Zevallos Ramos, Marlon Armando Carhuaz Matias, Axel Elian Quispe Romero, Marko Antonio Lengua Fernandez
| Received | Revised | Accepted | Published |
|---|---|---|---|
| 20 Mar 2026 | 19 Apr 2026 | 18 May 2026 | 30 Jun 2026 |
Citation :
Rike Ricardo Zevallos Ramos, Marlon Armando Carhuaz Matias, Axel Elian Quispe Romero, Marko Antonio Lengua Fernandez, "Self-Healing and Biological CO₂ Capture in Concrete Using Microencapsulated Nostoc Sphaericum," International Journal of Civil Engineering, vol. 13, no. 6, pp. 257-277, 2026. Crossref, https://doi.org/10.14445/23488352/IJCE-V13I6P118
Abstract
An increase in atmospheric CO₂ coupled with concrete production presents dual challenges in modern civil engineering that threaten structural integrity and contribute to global warming. In the Andean country of Peru, where the climate is highly variable, these challenges are exacerbated by the lack of sustainable technologies both to (i) repair existing structures and (ii) mitigate CO₂ emissions associated with cementitious materials. This study evaluated the effectiveness of microencapsulated Nostoc sphaericum in both bio-mortar and concrete with respect to self-healing and CO₂ biofixation. A total of six mixes were designed with five levels of microencapsulated Nostoc sphaericum (1.75%, 2.00%, 2.25%, 2.50%, and 2.75%) and a control without additives, with a target strength of 210 kg/cm². The experimental program included the determination of workability, compressive strength, and indirect tensile strength, crack Control, and gravimetric CO₂ uptake under controlled light and humidity conditions. The 1.75% mixture exhibited the best overall performance, with an increase in compressive strength of 0.8% compared to the Control, crack closure of 85.3%, and CO₂ biofixation of 2,583 mg CO₂/cm³ (4.05 g per test tube). The increase in dosage after 1.75% was recognised a limitation because of biopolymer saturation, also due to light diffusion. The work shows how a nutritious microencapsulated cushuro is designed to generate living, self-healing, and carbon-negative concrete without the addition of external nutrients. Such an innovative biotechnological output represents a disruptive and sustainable option for the Peruvian civil construction sector, by generating higher-performance materials with a reduced environmental impact.
Keywords
Biological Co₂ Capture, Biocementitious Concretes, Calcium Carbonate, Nostoc Sphaericum, Self-Repairing Concretes.
References
- Xiou Ge et al., “Accelerated Discovery of Sustainable Building Materials,” arXiv preprint, pp. 1-6, 2019.
[CrossRef] [Google Scholar] [Publisher Link] - Concrete Construction Materials Market Size, Global Growth Insights, 2025. [Online]. Available: https://www.globalgrowthinsights.com/market-reports/concrete-construction-materials-market-117910
- Huawei Li et al., “Lifetime Prediction of Damaged or Cracked Concrete Structures: A Review,” Structures, vol. 71, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Jiwei Ma et al., “Review on Durability Deterioration and Mitigation of Concrete Structures,” Coatings, vol. 15, no. 9, pp. 1-28, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Mônica Regina Garcez, and Nicola Tarque, “Building Durability, Climate Change and the Construction Life Cycle,” Journal of Building Pathology and Rehabilitation, vol. 10, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Muhammad Kabir et al., “Climate Change Due to Increasing Concentration of Carbon Dioxide and its Impacts on Environment in 21st Century; A Mini Review,” Journal of King Saud University Science, vol. 35, no. 5, pp. 1-7, 2023.
[CrossRef] [Google Scholar] [Publisher Link] - Lidong Li et al., “Global Greenhouse Gas Emissions from Agriculture: Pathways to Sustainable Reductions,” Global Change Biology, vol. 31, no. 1, pp. 1-11, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Songxi Yang et al., “The Rising Impact of Urbanization-caused CO2 Emissions on Terrestrial Vegetation,” Ecological Indicators, vol. 148, pp. 1-17, 2023.
[CrossRef] [Google Scholar] [Publisher Link] - Liming Huang et al., “Cement and Concrete as Carbon Sinks: Transforming a Climate Challenge into a Carbon Storage Opportunity,” Carbon Capture Science & Technology, vol. 16, pp. 1-13, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Kefei Li, and Le Li, “Crack-Altered Durability Properties and Performance of Structural Concretes,” Cement and Concrete Research, vol. 124, 2019.
[CrossRef] [Google Scholar] [Publisher Link] - S. Asvitha Valli, and M.S. Ravi Kumar, “Review on the Mechanism and Mitigation of Cracks in Concrete,” Applications in Engineering Science, vol. 16, pp. 1-9, 2023.
[CrossRef] [Google Scholar] [Publisher Link] - Concrete Repair Mortars Market Report 2026, The Business Research Company, 2026. [Online]. Available: https://www.thebusinessresearchcompany.com/report/concrete-repair-mortars-global-market-report
- Radek Ševˇcík et al., “Microcrack and Porosity Development in Sealed Cement Mortars Measured with Micro-Computed Tomography,” Materials, vol. 17, no. 13, pp. 1-14, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Ali Saberi Varzaneh, and Mahmood Naderi, “Determination of Shrinkage, Tensile and Compressive Strength of Repair Mortars and their Adhesion on the Concrete Substrate Using "Twist-off" and "Pull-off" Methods,” Iranian Journal of Science and Technology - Transactions of Civil Engineering, vol. 45, no. 4, pp. 2377-2395, 2021.
[CrossRef] [Google Scholar] [Publisher Link] - Tae-Kyun Kim, and Jong-Sup Park, “Experimental Evaluation of the Durability of Concrete Repair Materials,” Applied Sciences, vol. 11, no. 5, pp. 1-18, 2021.
[CrossRef] [Google Scholar] [Publisher Link] - Chunxiang Qian et al., “Self-healing of Cracks in Cement-based Materials through Bio-mineralization of Low Air-dependency Microorganisms,” Cement and Concrete Composites, vol. 154, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - A. Serag Farid et al., “Influence of Fascinating Diatom Fragilaria and Synechocystis Cyanobacteria on the Permeation Performance, Mechanical Properties, and Self-Healing Abilities of Concrete Under Curing Fresh Water and Seawater,” International Journal of Concrete Structures and Materials, vol. 19, no. 1, pp. 1-26, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - M. Karthick Srinivas et al., “Evaluation of Crack Healing Potential of Cement Mortar Incorporated with Blue-Green Microalgae,” Journal of Building Engineering, vol. 44, 2021.
[CrossRef] [Google Scholar] [Publisher Link] - Angelo J. Victoria, Michael J. Astbury, and Alistair J. McCormick, “Engineering Highly Productive Cyanobacteria Towards Carbon Negative Emissions Technologies,” Current Opinion in Biotechnology, vol. 87, pp. 1-9, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - David Choque-Quispe et al., “Preliminary Characterization of a Spray‑Dried Hydrocolloid from a High Andean Algae (Nostoc Sphaericum),” Foods, vol. 11, no. 11, pp. 1-15, 2022.
[CrossRef] [Google Scholar] [Publisher Link] - Christina Vasiliki Lazaratou et al., “Dynamics of Cyanobacteria/ Bacterial Consortia for CO2 Fixation and Simultaneous Wastewater Treatment,” Journal of Environmental Chemical Engineering, vol. 12, no. 6, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Fernandez Leon Jean Carlos et al., “Evaluation of the Compressive Strength of Concrete with Cushuro as a Natural Additive,” Thesis, Private University of the North, 2025.
[Publisher Link] - Nisha Rokaya et al., “Design of Co-culturing System of Diazotrophic Cyanobacteria and Filamentous Fungi for Potential Application in Self-Healing Concrete,” Materials Today Communications, vol. 44, pp. 1-12, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Mostafa Sobhi et al., “Environmental and Economic Assessment of Nitrogen-Fixing Nostoc sp. as a Sustainable Alternative to Synthetic Urea Fertilization,” Process Safety and Environmental Protection, vol. 196, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - N. Chasquibol et al., “Extraction and Characterization of Cushuro (Nostoc Sphaericum) Polysaccharides,” LWT, vol. 230, pp. 1-11, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Jaime Alberto Aguilar de La Cruz, and Luz Katherine Anchayhua Cayllahua, “Bioremediation of Soils Contaminated by Hydrocarbons using the Cyanobacterium Nostoc Sphaericum in Lurín - 2019,” Degree Thesis, Cesar Vallejo University, pp. 1-153, 2019.
[Google Scholar] [Publisher Link] - Manuel Brenes-Álvarez et al., “A Nitrogen Stress-inducible Small RNA Regulates CO2 Fixation in Nostoc,” Plant Physiology, vol. 187, no. 2, pp. 787-798, 2021.
[CrossRef] [Google Scholar] [Publisher Link] - Balamurali Kanagaraj et al., “Bamboo Reinforced Panels with Recycled Concrete Aggregates: A Comparative Study between Cement and Geopolymer Concrete,” Developments in the Built Environment, vol. 24, pp. 1-13, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Manuel Bustillo Revuelta, Concrete, Construction Materials: Geology, Production and Applications, Springer Cham, pp. 217-274, 2021.
[CrossRef] [Google Scholar] [Publisher Link] - N.E. 060, Reinforced Concrete, National Building Code, Government of Peru, pp. 1-205, 2009. [Online]. Available: https://drive.google.com/file/u/1/d/19EYUVMgwvm6rDs47GV374avco2ylU5Kz/view?usp=sharing&usp=embed_facebook
- Ying Peng, Xiu-Cheng Zhang, and Xue-Fei Chen, “Impact of Fractal Dimension on Recycled Aggregate Concrete Performance: Relationship with Damage Variables and Flexural Capacity Calculation of Normal Sections via Fractal-based Crack Analysis,” Journal of Materials Research and Technology, vol. 39, pp. 3007-3020, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - ACI Committee, 224R-01 Control of Cracking in Concrete Structures, pp. 1-46, 2001.
[Google Scholar] [Publisher Link] - Claudia Galarreta-Morales et al., “Sensory Development of Whey-based Yogurt with Cushuro (Nostoc Sphaericum), through Novel Consumer-based Sensory Evaluation Methods,” International Dairy Journal, vol. 166, pp. 1-10, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - N. Chasquibol et al., “Evaluation of Cushuro (Nostoc Sphaericum) as an Alternative Source of Minerals, Functional Protein and Bioactive Peptides,” LWT, vol. 217, pp. 1-9, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Yakov Felipe Carhuarupay-Molleda et al., “A Study of Methylene Blue Adsorption by a Synergistic Adsorbent Algae (Nostoc Sphaericum) Activated Clay,” Polymers, vol. 17, no. 15, pp. 1-24, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Nancy Chasquibol et al., “Co-Microencapsulation of Cushuro (Nostoc Sphaericum) Polysaccharide with Sacha Inchi Oil (Plukenetia Huayllabambana) and Natural Antioxidant Extracts,” Antioxidants, vol. 13, no. 6, pp. 1-18, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Trupti Sharma, Anirban Banerjee, and Prakash Nanthagopalan, “Probing the Abyss: Bacteria-based Self-Healing in Cementitious Construction Materials – A Review,” Construction and Building Materials, vol. 455, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Bo-Yu Peng et al., “Biodegradation of Polystyrene and Low-Density Polyethylene by Zophobas Atratus Larvae: Fragmentation into Microplastics, Gut Microbiota Shift, and Microbial Functional Enzymes,” Journal of Cleaner Production, vol. 367, 2022.
[CrossRef] [Google Scholar] [Publisher Link] - Luis Daniel Daza et al., “Study of the Physicochemical Properties of Hass Avocado Oil Encapsulated by Complex Coacervation,” LWT, vol. 204, pp. 1-10, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Callum White, and Janet M. Lees, “The Concrete Slump Test-A Rheometer by Definition?,” Construction and Building Materials, vol. 462, pp. 1-16, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Michael Autischer et al., “Improved Rheological Characterisation of Self-compacting Cementitious Pastes and Concrete by Advanced Slump Flow Test Analysis,” Construction and Building Materials, vol. 452, pp. 1-19, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Bo Wu, and DaoZhong Wu, “Test Study on Hydration Temperature of Compound Concrete Made of Demolished Concrete Lumps and Fresh Concrete,” Procedia Engineering, vol. 210, pp. 120-125, 2017.
[CrossRef] [Google Scholar] [Publisher Link] - Svenja Vogt, Felix Brück, and Harald Weigand, “Design and Test of a Novel Three-stage Batch Reactor for the Accelerated Carbonation of Fresh Concrete Slurry Waste,” Results in Engineering, vol. 27, pp. 1-9, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Wanmao Zhang, Dunwen Liu, and Kunpeng Cao, “Prediction of Concrete Compressive Strength using Support Vector Machine Regression and Non-Destructive Testing,” Case Studies in Construction Materials, vol. 21, pp. 1-20, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Ahmed Bensaber et al., “The Assessment of Concrete Subjected to Compressive and Flexural Preloading using Nondestructive Testing Methods, Correlation between Concrete Strength and Combined Method (SonReb),” Measurement, vol. 222, pp. 1-23, 2023.
[CrossRef] [Google Scholar] [Publisher Link] - Prayush Rajbhandari et al., “Tensile Performance of Headed Fasteners in High-Strength Concrete: Experimental and Numerical Analysis,” Construction and Building Materials, vol. 489, pp. 1-22, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Sagar Acharya, Mohamed A. Moustafa, and Shahrukh Shoaib, “Tensile Characteristics of Polymethyl Methacrylate Polymer Concrete under Different Strain Rates,” Case Studies in Construction Materials, vol. 22, pp. 1-15, 2025.
[CrossRef] [Google Scholar] [Publisher Link] - Muhammad Arslan Ahmad et al., “Synergistic Effect of Composite Bacteria on Self-healing Process of Concrete Crack,” Case Studies in Construction Materials, vol. 20, pp. 1-14, 2024.
[CrossRef] [Google Scholar] [Publisher Link] - Yifan Xing et al., “Roles of Illumination on Distribution of Phosphorus in Chlorella Vulgaris under Mixotrophic Cultivation,” Chemosphere, vol. 303, 2022.
[CrossRef] [Google Scholar] [Publisher Link] - Atrayee Bandyopadhyay et al., “Microbial Repairing of Concrete & Its Role in CO2 Sequestration: A Critical Review,” Beni-Suef University Journal of Basic and Applied Sciences, vol. 12, no. 1, pp. 1-13, 2023.
[CrossRef] [Google Scholar] [Publisher Link]