Design of Novel Laccases for Degradation of Complex Dyes

International Journal of Communication and Media Science

© 2019 by SSRG - IJCMS Journal

Volume 6 Issue 3

Year of Publication : 2019

Authors : Savita A.Patil, Sudarshan Shelke, Jyoti Kondhalkar, Shweta Dhumal, Amol Thete , Jagruti Lokare

10.14445/2349641X/IJCMS-V6I3P104

How to Cite?

Savita A.Patil, Sudarshan Shelke, Jyoti Kondhalkar, Shweta Dhumal, Amol Thete , Jagruti Lokare, "Design of Novel Laccases for Degradation of Complex Dyes," SSRG International Journal of Communication and Media Science, vol. 6,  no. 3, pp. 23-30, 2019. Crossref, https://doi.org/10.14445/2349641X/IJCMS-V6I3P104

Abstract:

Laccases also named p-diphenol: dioxide oxidoreductases are blue multicopper oxidizes (MCOs) that have the ability to catalyze the oxidation of a wide variety of organic aromatic compounds, concomitantly with the reduction of molecular oxygen to water. The present study is to isolate, extract purify and use the lacasse enzyme for degradation of complex dyes from Trichoderma viridae fungi. The fungi was isolated and maintained on PDA plates with multiple subcultures. Enzyme produced by two types of fermentation SSF and SMF. The solid state fermentation process by using wheat bran as solid support gives high production of enzyme. Screening and enzyme activity checked by using guaiacol as substrate this efficient substrate for laccase enzyme. The laccase enzyme shows the optimum activity at 350C and 4.00 pH. The activity was inhibited by 50% of organic solvents. Different dyes were checked for degradation by enzyme. The arylmethane group shows the high degradation percentage. The laccases are demonstrated to have a range of promising applications, they are used in bioremediation of soils, water and the development of environmentally friendly processes in the pulp and paper industry.
 

Keywords:

Laccases,  guaiacol, SDS, ammonium sulphate precipitation, oxidoreductases, bioremediation, decolorization, effluent treatment

References:

[1] Ducros V, Brzozowski AM, Wilson KS, et al. Nat. Struct. Biol. 1998, 5:310-316.
[2] Jolivalt C, Raynal A, Caminade E, et al. Appl. Microbiol. Biotechnol. 1999, 51:676-681. [3] Xu F. Biochem. 1996, 35:7608-7614.
[3] Thurston CF. Microbiol.1994 140:19-26
[4] Sterjiades R, Dean JF, Eriksson KL. Plant Physiol. 1992, 99:1162-1168
[5] Liu L, Dean JF, Friedman WE, Eriksson KEL. Plant J.1994, 6:213-224.
[6] Boudet AM. Plant Physiol. Biochem. 2000, 38:81-96.
[7] Ranocha P, Chabannes M, Chamayou S, et al. Plant Physiol. 2002, 129:145-155.
[8] Hoopes JT, Dean JFD. Plant Physiol. 2004, 42:27-33.
[9] Alexandre G, Zhulin IB. Trends Biotechnol. 2000, 18:41-42.
[10] Martins LO, Soares CM, Pereira MM, et al. J. Biol. Chem. 2002, 277:18849-18859.
[11] Claus H. Arch Microbiol. 2003, 179:145-150.
[12] Givaudan A, Effosse A, Faure D, et al. FEMS Microbiol Lett. 2004, 108:205-210
[13] Kiiskinen LL, Ratto M, Kruus K. J. Appl. Microbiol. 2004, 97:640-646.
[14] Bourbonnais R, Paice MG. FEBS Lett. 1990, 267:99-102.
[15] Shanmugam S, Rajasekaran P, Joseph-Thanikal V. Desalination and Water Treatment 2009, 4:143-147.
[16] Toca-Herrera JL, Osma JF, Rodriguez-Couto S. In:Communicating Current Research and Educational Topics and Trends in Applied Microbiology, Méndez-Vilas (Ed.), Formatex Research Center, Badajoz, Spain, 2007, 391-400.
[17] S. Sadhasivam, S. Savitha, K. Swaminathan, F.H. Lin. “Production, purification and characterization of mid-redox potential laccase from a newly isolated Trichoderma harzianum WL1”. Process Biochem. 2008, 43: 736-742.
[18] M.S. Revankar, S.S. Lele. “Enhanced production of laccase using a new isolate of white rot fungus WR- 1”. Proc. Biochem., 2006, 41: 581-588.
[19] L.L. Kiiskinen, M. Ratto and K. Kruus. “ Screening for novel laccase-producing microbes” .J. Appl. Microbiol., 2004, 97: 640.
[20] D.S. Arora, R.K. Sharma “ Ligninolytic Fungal Laccases and Their Biotechnological Applications” . Appl Biochem Biotechnol. 2010, 160:1760– 1788,
[21] M.R. Bailey, S.L. Woodard , E. Callawy, K.Beifuss, M.M. Lundback, J. Lane. “ Improved recovery of active recombinant laccase from maize seed” . Appl Microbiol Biotechnol. 2004, 63: 390– 397.
[22] P. Ranocha, M. Chabannes, S. Chamayou, S. Danoun, A. Jauneau, A.M. Boudet. “ Laccase down regulation causes alteration in phenolic metabolism and cell wall structure in poplar” . Plant Physiol. 2002, 129:1– 11.
[23] A.Givaudan, A. Effose, D. Faure, P. Potier, M.L. Bouillant, R. Bally. “ Polyphenol oxidize in Azospirillum lipoferum isolated from rice rhizosphere: evidence for laccase activity in nonmotile strains of Azospirillum lipoferum. FEMS Microbiol Lett. 1993, 108: 205– 210.
[24] K.K. Sharma and R.C. Kuhad “ Laccase: enzyme revisited and function redefined” . Ind J Microbiol. 2008 48: 309–316.
[25] G.Alexandre and I.B. Zhulin “ Laccases are wide spread in bacteria” . Trends in Biotech. 2000 18: 41– 42.
[26] R. Bourbonnais, M.G. Paice, I.D. Reid, P. Lanthier & M. Yaguchi, “ Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2' –Azinobis (3- Ethylbenzthiazoline-6- Sulfonate) in kraft lignin depolymerization” . Appl. Environ. Microbiol. 1995, 61(5): 1876-1880.
[27] R. Bourbonnais and M. G. Paice, “ Oxidation of nonphenolic substrates. An expanded role for laccase in lignin biodegradation,” FEBS Letters, 1990, vol. 267, no.1, pp. 99– 102.
[28] K.Brijwani, A.Rigdon, P.V. Vadlani, Fungal Laccase production ,Functions and Application in Food Processing, Enzyme Research, volume 2010 (2010) article id 149748, 10 pages.
[29] V. K. Gochev and A. I. Krastanov, “ Fungal laccases,” Bulgarian Journal of Agricultural Science, 2007.,13, pp.75–83.
[30] E. Grassi, P. Scodeller, N. Filiel, R. Carballo, and L. Levin, "Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators," International Biodeterioration andBiodegradation, vol. 65,pp. 635-643, 2011.
[31] I. Ciullini, S. Tilli, A. Scozzafava, and F. Briganti, "Fungal laccase, cellobiose dehydrogenase, and chemical mediators: Combined actions for the decolorization of different classes of textile dyes," BioresourceTechnology, vol. 99, pp. 7003-7010, 2008.
[32] T. Mechichi, N. Mhiri, and S. Sayadi, "Remazol Brilliant Blue R decolourization by the laccase from Trametes trogii," Chemosphere, vol. 64, pp. 998-1005, 2006.
[33] H. R. K. Hamedaani, A. Sakurai, and M. Sakakibara, "Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus," Dyes and Pigments,vol. 72, pp. 157- 162, 2007.
[34] Kiiskinen Et Al. [2004]. And Decolorization And Detoxification Of Textile Dyes With A Laccase From Trametes Hirsute Elias Abadulla et al, applied and environmental microbiology, 0099-2240/00/$04.0010 aug. 2000, p. 3357– 3362 vol. 66, no. 8
[35] ” Isolation Of Laccase Producing Trichoderma Spp. And EffectOf PH and Temperature on Its Activity” Kuntal Kalra et al /Int.J.ChemTech Res.2013, 5(5)
[36] Characterization of a Novel White Laccase from the Deuteromycete Fungus Myrothecium verrucaria NF 05and Its Decolourisation of Dyes, Dan Zhao et al June 2012 Volume 7, Issue 6, e38817
[37] “ Isolation of laccase producing fungi and partial characterization of Laccase” SavithaS et al. Research Article, Biotechnol. Bioinf. Bioeng. 2011, 1(4):543-549