Prevalence of Extended Spectrum β-Lactamases and Molecular Screening of klebsiella pneumoniae in the West Bank, Palestine

International Journal of Medical Science

© 2018 by SSRG - IJMS Journal

Volume 5 Issue 8

Year of Publication : 2018

Authors : Mohammad A.A. Shawabka and Gabi M.Abusada

10.14445/23939117/IJMS-V5I8P103

How to Cite?

Mohammad A.A. Shawabka and Gabi M.Abusada, "Prevalence of Extended Spectrum β-Lactamases and Molecular Screening of klebsiella pneumoniae in the West Bank, Palestine," SSRG International Journal of Medical Science, vol. 5,  no. 8, pp. 9-17, 2018. Crossref, https://doi.org/10.14445/23939117/IJMS-V5I8P103

Abstract:

Klebsiella are Gram negative, non-motile, rod-shaped, lactose-fermenting, facultative anaerobic bacteria and encapsulated with a polysaccharide capsule. It causes opportunistic and nosocomial infections. Klebsiella produce plasmid-mediated extended-spectrum beta lactamases (ESBLs) which gives the bacteria the ability to resist beta-lactam antibiotics. The prevalence of ESBLs among clinical isolates of Klebsiella pneumonia was determined. In addition, the specific molecular characterization of CTX-M genes were also determined. Sixty seven urine, pus, and wound swabs were collected from patients of gynecology, surgery, medicine, Intensive Care Unit (ICU), and orthopedics from different parts of the West Bank of Palestine, mainly Ramallah, Hebron, and Jerusalem. Antibiotic sensitivity were done by combination disk method and double-disk synergy test. ESBL genes were detected by Polymerase Chain Reaction (PCR) and multiple PCR. Results showed that 48 samples were positive for CTX-M universal gene. Sixty four samples were positive for SHV gene, and 51 samples were positive for TEM gene. Forty two samples were positive for CTX-M1 gene, 38 samples were positive for CTX-M9 gene. Seven samples from 42 samples were positive for CTX-M1 only, 3 samples from 38 samples were positive for CTX-M9 only, and 35 samples were positive for both genes (CTX-M1 and CTX-M9). Our results were close to several studies around the world mainly in Egypt and Israel. In conclusion, bacteria are becoming more complex and highly spread in hospitals and the community in Palestine.

Keywords:

ESBLs, Intensive Care Unit, Klebsiella, PCR, CTX-M

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