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The Effectiveness of Glass Bead and Glutaraldehyde in Sterilizing the Endodontic Files Contaminated with Aerobic Microorganism: A Comparative Study

International Journal of Medical Science
© 2023 by SSRG - IJMS Journal
Volume 10 Issue 4
Year of Publication : 2023
Authors : Md. Tarik Immam Hossain Molla, Shinthia Masud, Rezaul Kabir, Md. Ashraf Ali, Mozammal Hossain
10.14445/23939117/IJMS-V10I4P101
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Md. Tarik Immam Hossain Molla, Shinthia Masud, Rezaul Kabir, Md. Ashraf Ali, Mozammal Hossain, "The Effectiveness of Glass Bead and Glutaraldehyde in Sterilizing the Endodontic Files Contaminated with Aerobic Microorganism: A Comparative Study," SSRG International Journal of Medical Science, vol. 10,  no. 4, pp. 1-7, 2023. Crossref, https://doi.org/10.14445/23939117/IJMS-V10I4P101

Abstract:

In order to maintain asepsis and avoid cross-contamination from one person to another or tooth to another, sterilizing endodontic equipment is essential. In this work, the effectiveness of glass beads and glutaraldehyde in sterilizing endodontic files contaminated with infected pulp and dentinal tissue is assessed and compared. The diseased root canal contaminated samples of endodontic k-files. The samples were then aerobically delivered to the lab. It was determined that the files were microbiologically contaminated by incubating the files for 72 hours under aerobic conditions. The files were divided into three groups and sterilized as necessary while being carefully cleaned, dried, and sterilized using glass beads, glutaraldehyde, and an autoclave (as a control). After the instrument shaft was detached from the handle using a sterile autoclaved wire cutter, all sanitized endodontic K-files were put in separate test tubes containing 2.5 mL of Buffered Peptone Water to look for any microbial growth. Vortexing was followed by surface plating on Tryptic Soy Agar (TSA) plates, which were then incubated for 72 hours under aerobic conditions. Colony counting was used to assess the efficiency of the endodontic file sterilization methods. The findings showed that 6 of 31 (19.4%) files sterilized with glass beads and 7 of 31 (22.6%) samples sterilized with glutaraldehyde solution showed signs of microbial growth. The samples that went through autoclaving (the control) showed no signs of microbial development. Furthermore, there was no statistically significant difference in the sterilizing effectiveness of glass beads and glutaraldehyde. It was established that the endodontic files contaminated with infected pulp and dentinal tissue may be sterilized using glass beads and glutaraldehyde with comparable success.

Keywords:

Sterilization, Glass bead, Glutaraldehyde, Colony, Aerobic microorganism.

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