Killing Effect of IL-13Rα2 Sensitized DC-CTL Cells on Mice Glioma in Vivo

International Journal of Medical Science

© 2016 by SSRG - IJMS Journal

Volume 3 Issue 4

Year of Publication : 2016

Authors : Ming Tu

10.14445/23939117/IJMS-V3I4P102

How to Cite?

Ming Tu, "Killing Effect of IL-13Rα2 Sensitized DC-CTL Cells on Mice Glioma in Vivo," SSRG International Journal of Medical Science, vol. 3,  no. 4, pp. 5-7, 2016. Crossref, https://doi.org/10.14445/23939117/IJMS-V3I4P102

Abstract:

Abstract: To discuss the killing effect of IL-13Rα2 sensitized DC-CTL cells on mice glioma in vivo. Peripheral blood mononuclear cells (PBMC) of mice were isolated by Ficoll density gradient centrifugation and were cultured with rmGM-CSF, rmIL-4 and TNF-α into DCs in vitro. On the fifth day of being cultured by U251 cells, DCs was added with TNF-α and then co-cultured with the T cells from mice spleen to induce antigen-specificity CTL of U251 cells. Mice were given U251 cells by subcutaneous injection to prepare C57BL/6 glioma models, which were randomly divided into physiological saline (NS) group, DC group and IL-13Rα2-DC-CTL group. Weights of tumors, tumor volumes of mice in three groups were respectively measured on the ninth day of mice bearing tumors.. Meanwhile, tumor growth curves were drawn and death cases of mice were observed. The average tumor volumes of mice respectively were 3.15 ± 0.3cm3 (NS group), 2.97 ± 0.29 cm3 (DC group) and 2.21 ± 0.25 cm3 (IL-13Rα2-DC-CTL group) measured on the ninth day of bearing cancer, as the comparison among the tumor volumes of mice in three groups was statistically significant (p<0.001): the volume growth for IL-13Rα2-DC-CTL group was slower than that of the rest two groups (p<0.01); IL-13Rα2 antigen sensitized DC-CTL had a killing effect on glioma cells in vivo, which provided a new direction for the targeting immunotherapy of glioma.

Keywords:

IL-13Rα2, Glioma, Immune targeted therapy.

References:

[1] Jeremias I ,Steiner H H ,Benner A ,et al . Cell death induction by etulinic acid , ceramide and TRAIL in primary glioblastoma multiforme cells. Acta Neurochir (Wien) ,2004 ,146(7) :721 - 729.
[2] Singh SK, Clarke ID, Terasaki M, et al.Identification of a cancer stem cell in human brain tumors. Cancer Res, 2003,63(18):5821-5828.
[3] Xiaobing J, Xiaoling L, Ruen L, et al.Induction of cytotoxic T-lymphocytes specific for malignant glioma by HLA dimer-based artificial antigen-presenting cells. Cancer Biother Radiopharm. 2007 Dec,22(6):826-835.