Preparation and Evaluation of Biocompatible Composite for Bone Tissue Engineering

International Journal of Pharmacy and Biomedical Engineering

© 2017 by SSRG - IJPBE Journal

Volume 4 Issue 3

Year of Publication : 2017

Authors : Md. Masud Rana, Naznin Akhtar, Md. Zahid Hasan and S.M. Asaduzzaman

10.14445/23942576/IJPBE-V4I3P102

How to Cite?

Md. Masud Rana, Naznin Akhtar, Md. Zahid Hasan and S.M. Asaduzzaman, "Preparation and Evaluation of Biocompatible Composite for Bone Tissue Engineering," SSRG International Journal of Pharmacy and Biomedical Engineering, vol. 4,  no. 3, pp. 8-15, 2017. Crossref, https://doi.org/10.14445/23942576/IJPBE-V4I3P102

Abstract:

Bone tissue engineering with cells and synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissue than bone transplantation. Hydroxyapatite (HA) and its composite with a biopolymer are extensively developed and applied in bone tissue regeneration. This study's main aim was to fabricate and characterize HA apatite-based biocompatible scaffold for bone tissue engineering. Scaffolds with different polymers (chitosan & alginate) ratios and a fixed amount of synthetic HA were prepared using in situ co-precipitation method. The mineral to polymer ratio was 1:1 to 1: 2. A crosslinker agent, 2-Hydroxylmethacrylate (HEMA), was added at a different percentage (0.5-2%) into the selected composition and irradiated at 5- 25 kGy to optimize the proper mixing of components at the presence of HEMA. Fabricated scaffolds were analyzed to determine porosity, density, biodegradability, morphology, and structural properties. The prepared scaffold's porosity and density were 75 to 92% and 0.21 to 0.42 g/cm3, respectively. However, the swelling ratio of the fabricated scaffolds was ranged from 133 to 197%. Nonetheless, there had a reasonable in-vitro degradation of prepared scaffolds. Flourier transform infrared spectroscopy (FTIR) analysis showed intermolecular interaction between components in the scaffold. A scanning electron microscope measured the scaffold's pore size, and the value was 162-510 µm. It could be proposed that this scaffold fulfills all the main requirements to be considered as a bone substitute for biomedical applications shortly.

Keywords:

Bone tissue engineering, Scaffold, Chitosan, Alginate, Hydroxyapatite.

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