Prevalence of Toxoplasmosis among Antenatal Patients Attending the Outpatient Department of a Tertiary Care Health Facility

International Journal of Pharmacy and Biomedical Engineering

© 2025 by SSRG - IJPBE Journal

Volume 12 Issue 2

Year of Publication : 2025

Authors : Mzhda Adam Waseem, Meera Sujeet Modak

10.14445/23942576/IJPBE-V12I2P102

How to Cite?

Mzhda Adam Waseem, Meera Sujeet Modak, "Prevalence of Toxoplasmosis among Antenatal Patients Attending the Outpatient Department of a Tertiary Care Health Facility," SSRG International Journal of Pharmacy and Biomedical Engineering, vol. 12,  no. 2, pp. 8-17, 2025. Crossref, https://doi.org/10.14445/23942576/IJPBE-V12I2P102

Abstract:

Acute infection with Toxoplasma gondii during early pregnancy presents a substantial risk of transplacental transmission, which may lead to severe fetal complications. Detecting specific IgG and IgM antibodies through serological assays is critical for differentiating recent infections from prior exposures. This cross-sectional study involved 50 pregnant women across all three trimesters who attended an antenatal care (ANC) clinic in India. Serum samples were assessed for T. gondii-specific IgG and IgM antibodies using two automated platforms: the VITEK Immunodiagnostic Assay System (VIDAS, employing the ELFA method) and the Architect i2000 system (utilizing the CMIA method). Of the participants, 18% (9 out of 50) were seropositive for T. gondii-specific IgG antibodies (P = 0.038). Seropositivity was most prevalent in the third trimester (66.66%), significantly higher than in the first and second trimesters (11.11%) (P < 0.05). A higher rate of IgG positivity was also observed among non-vegetarian participants (77.77%) compared to vegetarians (22.22%) (P = 0.272), and among primigravida women (55.55%) compared to multigravida women (22.22%) (P = 0.735). In conclusion, both the Architect i2000 (CMIA) and VIDAS TOXOM (ELFA) systems demonstrated high sensitivity and specificity, confirming their utility in the serodiagnosis of toxoplasmosis among pregnant women, particularly those with adverse obstetric histories. The study also emphasizes the need to improve expectant mothers' awareness of transmission risks. Targeted educational programs and preventive counselling could play a crucial role in mitigating the incidence of congenital toxoplasmosis. Furthermore, public health initiatives should aim to control T. gondii infections in livestock, particularly sheep and goats, which serve as key zoonotic sources.

Keywords:

Toxoplasma gondii, Trimesters of pregnancy, Serological immunoassay test, Technical automated system, CLIA, VIDAS.

References:

[1] Rebaz Ramadhan Abdulrahman et al., “The Role of Agriculture Sector in Eradicating Poverty: Challenges, Policies, and Pathways for Economic Growth in Less Developed Countries,” International Journal of Social Science and Education Research Studies, vol. 5, no. 2, pp. 208-213, 2025.
[CrossRef] [Google Scholar] [Publisher Link]
[2] Shwan Adam Aivas et al., “Critically Discuss Epistemological Issues by Examining the Claim to ‘Truth’,” International Journal of Social Science and Education Research Studies, vol. 5, no. 1, pp. 78-83, 2025.
[CrossRef] [Google Scholar] [Publisher Link]
[3] Brogen Singh Akoijam et al., “Seroprevalence of Toxoplasma Infection among Primigravid Women Attending Antenatal Clinic at a Secondary Level Hospital in North India,” Journal of the Indian Medical Association, vol. 100, no. 10, 2002.
[Google Scholar] [Publisher Link]
[4] Adel Almogren, “Antenatal Screening for Toxoplasma Gondii Infection at a Tertiary Care Hospital In Riyadh, Saudi Arabia," Annals Saudi Medicine, vol. 31, no. 6, pp. 569- 572, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[5] J.P. Allain, C.R. Palmer, and G. Pearson, “Epidemiological Study of Latent and Recent Infection by Toxoplasma Gondii in Pregnant Women from a Regional Population in the UK,” Journal of Infection, vol. 36, no. 2, pp. 189-96, 1998.
[CrossRef] [Google Scholar] [Publisher Link]
[6] Nuha Awat Aziz, and Photo Shwan Adam Aivas, “Consumer Satisfaction in Kurdish Online Shopping: Kurdistan Region of Iraq as a Case Study,” International Journal of Scientific Research and Technology, vol. 2, no. 3, pp. 239-251, 2025.
[CrossRef] [Google Scholar] [Publisher Link]
[7] A.H. Balfour et al., “Comparative Study of Three Tests (Dye Test, Indirect Haemagglutination Test, Latex Agglutination Test) for the Detection of Antibodies to Toxoplasma Gondii in Human Sera,” Journal of Clinical Pathology, vol. 35, no. 2, pp.228-232, 1982.
[CrossRef] [Google Scholar] [Publisher Link]
[8] Michael W. Black, and John C. Boothroyd, “Lytic Cycle of Toxoplasma Gondii,” Microbiology and Molecular Biology Reviews, vol. 64, no. 3, pp. 607-623, 2000.
[CrossRef] [Google Scholar] [Publisher Link]
[9] M. Breugelmans, A. Naessens, and W. Foulon, Prevention of Toxoplasmosis during Pregnancy – An Epidemiologic Survey Over 22 Consecutive Years, vol. 32, no. 4, pp. 211-214, 2004.
[CrossRef] [Google Scholar] [Publisher Link]
[10] Anne O. Carter, and John W. Frank, “Congenital Toxoplasmosis: Epidemiologic Features and Control,” Canadian Medical Association Journal, vol. 135, pp. 618-623, 1986.
[Google Scholar] [Publisher Link]
[11] Suryamani Chintapalli, and I. Jyothi Padmaja, “Seroprevalence of Toxoplasmosis in Antenatal Women with Bad Obstetric History,” Tropical Parasitology, vol. 3, no. 1, pp. 62-66, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[12] A.J.C. Cook et al., “Sources of Toxoplasma Infection in Pregnant Women: European Multicentre Case-Control Study Commentary: Congenital Toxoplasmosis—Further Thought for Food,” BMJ, vol. 321, pp. 142-147, 2000.
[CrossRef] [Google Scholar] [Publisher Link]
[13] J.P. Dubey, and J.L. Jones, “Toxoplasma Gondii Infection in Humans and Animals in the United States,” International Journal for Parasitology, vol. 38, no. 11, pp.1257-1278, 2008.
[CrossRef] [Google Scholar] [Publisher Link]
[14] Jitender P. Dubey, “The History of Toxoplasma Gondii—the First 100 Years,” Journal of Eukaryotic Microbiology, vol. 55, no. 6, pp. 467-475, 2008.
[CrossRef] [Google Scholar] [Publisher Link]
[15] J.P. Dubey, Toxoplasmosis of Animals and Humans, CRC Press, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[16] Siala Emna et al., “Difficulty in dating primary infections by Toxoplasma gondii in pregnant women in Tunisia,” Medical Tunisia, vol. 84, no. 2, pp. 85-87, 2006.
[Google Scholar] [Publisher Link]
[17] Sandra K. Halonen, and Louis M. Weiss, Toxoplasmosis, Handbook of Clinical Neurology, vol. 114, pp.125-145, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[18] Jeffrey L. Jones et al., “Congenital Toxoplasmosis: A Review,” Obstetrical & Gynecological Survey, vol. 56, no. 5, pp. 296-305, 2001.
[Google Scholar] [Publisher Link]
[19] G.I. Kaufman, J.S. Remington, and H.C. Waters, “Automation of the Indirect Fluorescent-Antibody Test for Toxoplasmosis,” Applied Microbiology, vol. 25, no. 5, pp. 724-730, 1973.
[CrossRef] [Google Scholar] [Publisher Link]
[20] Morten Lebech, Severin Olesen Larsen, and Eskild Petersen, “Prevalence, Incidence and Geographical Distribution of Toxoplasma Gondii Antibodies in Pregnant Women in Denmark,” Scandinavian Journal of Infectious Diseases, vol. 25, no. 6, pp. 751-756, 1993.
[CrossRef] [Google Scholar] [Publisher Link]
[21] P. Mazumder et al., “Latex Agglutination Test for Detection of Antibodies to Toxoplasma Gondii,” Journal of Clinical Microbiology, vol. 26, no. 11, pp. 2444-2446, 1988.
[CrossRef] [Google Scholar] [Publisher Link]
[22] Balvinder Mohan et al., “Seroepidemiological Study of Toxoplasmosis in Different Sections of Population of Union Territory of Chandigarh,” The Journal of Communicable Diseases, vol. 34, no. 1, pp. 15-22, 2002.
[Google Scholar] [Publisher Link]
[23] Arthur Morris, and Margaret Croxson, “Serological Evidence of Toxoplasma Gondii Infection among Pregnant Women in Auckland,” New Zealand Medical Journal, vol. 117, no. 1189, pp. 1-6, 2004.
[Google Scholar] [Publisher Link]
[24] S.G. Parija, Coccidian Textbook of Medical Parasitology, 3rd Edition, New Delhi: All India Publishers and Distributors, 2009. [25] J.W. Peterson, Bacterial Pathogenesis, Medical Microbiology, 4th edition, 1996.
[Google Scholar]
[26] Amir Hossien Rahbari et al., “IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans,” The Korean Journal of Parasitology, vol. 50, no. 2, pp. 99-102, 2012.
[CrossRef] [Google Scholar] [Publisher Link]
[27] Myrian Morussi Reis, Maria Madalena Tessaro, and Pedro Alves D'Azevedo, “Toxoplasma-IgM and IgG-avidity in Single Samples from Areas with a High Infection Rate Can Determine the Risk of Mother-to-Child Transmission,” Journal of the Institute of Tropical Medicine of São Paulo, vol. 48, no. 28, pp. 93-98, 2006.
[CrossRef] [Google Scholar] [Publisher Link]
[28] I. Reiter-Owona et al., “The Past and Present Role of the Sabin-Feldman Dye Test in the Serodiagnosis of Toxoplasmosis,” Bulletin of the World Health Organization, vol. 77, no. 11, pp. 929-935, 1999.
[Google Scholar] [Publisher Link]
[29] Florence Robert-Gangneux, and Marie-Laure Dardé, “Epidemiology of and Diagnostic Strategies for Toxoplasmosis,” Clinical Microbiology Reviews, vol. 25, no. 2, pp. 264-296, 2012.
[CrossRef] [Google Scholar] [Publisher Link]
[30] Makiko Sakikawa et al., “Anti-Toxoplasma Antibody Prevalence, Primary Infection Rate, and Risk Factors in a Study of Toxoplasmosis in 4,466 Pregnant Women in Japan,” Clinical and Vaccine Immunology, vol. 19, no. 3, pp. 365-367, 2012.
[CrossRef] [Google Scholar] [Publisher Link]
[31] Ali Mohammed Salih et al., “Characterization of Natural Zeolite and Evaluation of Its Adsorption Capacity,” British Journal of Interdisciplinary Research, vol. 2, no. 4, pp. 31-47, 2025.
[CrossRef] [Google Scholar] [Publisher Link]
[32] Hassan Sanati et al., “Seroprevalence of Toxoplasma Gondii Antibodies in Dairy Cows in Kerman Province, South East Iran,” Current Research Journal of Biological Sciences, vol. 4, no. 4, pp. 417-421, 2012.
[Google Scholar] [Publisher Link]
[33] Sarman Singh, and Amol J. Pandit, “Incidence and Prevalence of Toxoplasmosis in Indian Pregnant Women: A Prospective Study,” American Journal of Reproductive Immunology, vol. 52, no. 4, pp. 276-283, 2004.
[CrossRef] [Google Scholar] [Publisher Link]
[34] S. Singh, “Mother-to-Child Transmission and Diagnosis of Toxoplasma Gondii Infection during Pregnancy,” Indian Journal of Medical Microbiology, vol. 21, no. 2, pp. 69-76, 2003.
[CrossRef] [Google Scholar] [Publisher Link]
[35] S. Singh et al., “Toxoplasma Gondii Infection & Its Association with Iodine Deficiency in a Residential School in a Tribal Area of Maharastra,” Indian Journal of Medical Research, vol. 99, pp. 27-31, 1994.
[Google Scholar]
[36] G. Sucilathangam et al., “Serological Survey of Toxoplasmosis in a District in Tamil Nadu: Hospital-Based Study,” Indian Journal of Medical Research, vol. 137, no. 3, pp. 560-563, 2013.
[Google Scholar] [Publisher Link]
[37] Vikrant Sudan, Amit K. Jaiswal, and Daya Shanker, “Recent Trends in the Diagnosis of Toxoplasmosis,” Clinical Reviews and Opinions, vol. 5, no. 2, pp. 11-17, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[38] E. Fuller Torrey, John J. Bartko, and Robert H. Yolken, “Toxoplasma Gondii and Other Risk Factors for Schizophrenia: An Update,” Schizophrenia Bulletin, vol. 38, no. 3, pp. 642-647, 2012.
[CrossRef] [Google Scholar] [Publisher Link]
[39] Gerard J. Tortora, Berdell R. Funke, and Christine L. Case, Microbiology An Introduction, Benjamin/Cummings Publishing Company, pp. 1-772, 1982.
[Google Scholar] [Publisher Link]
[40] Uysal, A., Cüce, M., Tañer, C.E., Uysal, F., Atalay, S., Göl, B. and Köse, S., “Prevalence of Congenital Toxoplasmosis among a Series of Turkish Women,” Medical Journal of Chile, vol. 141, no. 4, pp. 471-476, 2013.
[Google Scholar]
[41] F.A. Vinhal et al., “Analysis of Aqueous Humor in Ocular Toxoplasmosis: Detection of Low Avidity IgG Specific to Toxoplasma Gondii,” Applied Parasitology, vol. 35, no. 1, pp. 1-7, 1994.
[Google Scholar] [Publisher Link]
[42] Phuangphet Waree, Toxoplasmosis: Pathogenesis and Immune Response, 2010.
[Google Scholar] [Publisher Link]