Development And Validation Of RP-HPLC Method For Simultaneous Estimation of Piracetam and Mecobalamin in Bulk and its Pharmaceutical Formulations

International Journal of Applied Chemistry

© 2021 by SSRG - IJAC Journal

Volume 8 Issue 1

Year of Publication : 2021

Authors : B.Sucharitha, Prof.V. Anuradha

10.14445/23939133/IJAC-V8I1P104

How to Cite?

B.Sucharitha, Prof.V. Anuradha, "Development And Validation Of RP-HPLC Method For Simultaneous Estimation of Piracetam and Mecobalamin in Bulk and its Pharmaceutical Formulations," SSRG International Journal of Applied Chemistry, vol. 8,  no. 1, pp. 13-20, 2021. Crossref, https://doi.org/10.14445/23939133/IJAC-V8I1P104

Abstract:

A new, simple, rapid, selective, precise, and accurate isocratic reverse-phase high-performance liquid Chromatography assay method has been developed for simultaneous estimation of Piracetam and Mecobalamin tablet formulations. The separation was achieved by using column Phenomenex Luna C18, 250x4.6mm, 5μm, in mobile phase consisted of pH 6.0 phosphate buffer and Acetonitrile and methanol in the ratio of 40:50:10 v/v/v. The flow rate was 1.0 mL/min, column oven temperature 25° C, sample cooler temperature 25°C and the injection volume was 20μL, and detection was performed at 215 nm using a photodiode array detector (PDA), Run time 7minutes. The retention time of Piracetam and Mecobalamin was noted to be 2.218 minutes and 3.68 minutes, respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.

Keywords:

Liquid Chromatography, Piracetam, Mecobalamin, combined dosage forms, Simultaneous estimation, Validation

References:

[1] Babu G, Prasad K, Kolla T, Vijayabaskaran M, Latha S. Method development and Validation of RP-HPLC for simultaneous estimation of Citicoline and Piracetam in tablet dosage form, International Journal of PharmTech Research. 3(2011) 1311-1313.
[2] Jadhav RT, Patil PH, Patil PR. Formulation and evaluation of bilayered tablet of Piracetam and Vinpocetine. Journal of Chemical and Pharmaceutical Research. 3(3)(2011) 423-431.
[3] Shorvon S. Pyrrolidone derivatives. The Lancet. 358(9296)(2001) 1885-1892.
[4] Winblad B. Piracetam: a review of pharmacological properties and clinical uses. CNS drug reviews. 11(2)(2005) 169-182.
[5] Anindita AB, Khandelwal K, Deepali VM, Swapnil K. Analytical method development and Validation for piracetam as bulk and in a pharmaceutical formulation. International Journal of PharmTech Research. 2(2010) 201-204.
[6] Dhoru MM, Surani S, Mehta P. UV-Spectrophotometric methods for determination of citicoline sodium and piracetam in a pharmaceutical formulation. Der Pharmacia Lettre. 4(2012) 1547-1552.
[7] Pharmacopoeia I. Vol. 3. New Delhi: The Controller Publication, Govt of India. (2010) 1018.
[8] Pharmacopoeia B. The stationary office, MHRA. British Pharmacopoeial Commission office, London. 1(2005).
[9] Dwi Lestari A, Tri Prasetyo A, Palupi T, Umayah E, Yuwono M, Indrayanto G. HPLC Determination of piracetam in tablets; Validation of the method. Journal of liquid chromatography & related technologies. 28(9)(2005) 1407-1416.
[10] Arayne MS, Sultana N, Siddiqui FA, Mirza AZ, Qureshi F, Zuberi MH. Simultaneous determination of piracetam and its four impurities by RP-HPLC with UV Detection. Journal of chromatographic science. 48(7)(2010) 589-594.
[11] Bhadra S, Das SC, Roy S, Arefeen S, Rouf ASS. Development and validation of a RP-HPLC method for quantitative estimation of vinpocetine in pure and pharmaceutical dosage forms, Chromatography Research International. (2011) 1-7.
[12] El-Gindy A, Emara S, Mesbah MK, Hadad GM. Spectrophotometric and liquid chromatographic determination of fenofibrate and vinpocetine and their hydrolysis products. II Farmaco., 60(5)(2005) 425-438.
[13] Japanese Pharmacopoeia XIV Edition.,590-591.
[14] Lindstrand K. Isolation of methylcobalamin from the natural source material. Nature. 204(1964) 188-189.
[15] hrimant N Panaskar, Mahesh H Hampe, Mr. Susheel Singh, Akshata Shivalkar, Pallavi Kadam, A Fast & Reliable Liquid Chromatography Mass Spectrometry Method for Determination of Amino acids & Acylcarnitines including Succinyl acetone in dried blood spots for newborn screening. SSRG International Journal of Medical Science 6(7) (2019) 1-11.