Call For Paper - Upcoming Conferences

Research Article | Open Access | Download PDF
Volume 12 | Issue 2 | Year 2026 | Article Id. IJVS-V12I2P102 | DOI : https://doi.org/10.14445/24550868/IJVS-V12I2P102

Hill Lemon-Based Phytotherapy as a Promising Intervention for Teat Fibrosis in Cattle


Safdar Aman, Aneela Zameer Durrani, Shaukat Aman

Received Revised Accepted Published
09 May 2026 12 Jun 2026 30 Jun 2026 14 Jul 2026

Citation :

Safdar Aman, Aneela Zameer Durrani, Shaukat Aman, "Hill Lemon-Based Phytotherapy as a Promising Intervention for Teat Fibrosis in Cattle," International Journal of Veterinary Science, vol. 12, no. 2, pp. 9-17, 2026. Crossref, https://doi.org/10.14445/24550868/IJVS-V12I2P102

Abstract

Teat fibrosis, a significant health issue in cattle, compromises productive lifespan and milk quality, necessitating effective therapeutic interventions. This study investigates the therapeutic efficacy of Hill Lemon (Citrus pseudo lemon Tanaka) essential oil, both independently and in combination with other drugs like a traditional local recipe (Hill lemon extract), in treating teat fibrosis (teat hardness, stiffness, nodularity, and atrophy) among cattle in the Swat district, Khyber Pakhtunkhwa, Pakistan. The therapeutic trial was conducted on 32 cattle diagnosed with teat fibrosis, divided into four treatment groups designated as A, B, C and D. Group A received 1% hill lemon essential oil 10ml per affected teat IMM, Sid, for 14 days; Group B, a local recipe (Hill lemon extract) containing Hill Lemon pulp, sugar, Himalayan pink salt and ammonium chloride 500g on alternative days for 14 days (PO); Group C, a combination of Hill Lemon essential oil 10ml per affected teat IMM, Sid, for 14 days and the local recipe (Hill lemon extract) 500g on alternative days for 14 days (PO); and Group D, Hill Lemon essential oil 10ml per affected teat IMM, Sid, for 14 days scombined with the commercial preparation Mastifit (10ml IMM,60ml Bid PO). Each treatment protocol was administered over 14 days with assessments on days 0, 5, 10, 15, and 20. Recovery metrics for teat hardness, stiffness, nodularity, and atrophy indicated that Group C achieved the highest efficacy, showing nearly complete recovery across the first three parameters (100%) and partial recovery of the fourth parameter (90%) by day 20. The combination of Hill Lemon essential oil and the local recipe (Group C) emerges as a potent intervention for teat fibrosis in cattle, demonstrating superior recovery outcomes. These findings position Hill Lemon essential oil as a promising natural therapeutic agent for fibrotic mastitis management in cattle.

Keywords

Cattle, Mastitis, Teat Fibrosis, Hill Lemon, Essential Oils, Intra Mammary (IMM), Orally (PO), Sid (once in a day), Bid (twice a day).

References

  1. Diaa Alabed et al., “Novel Agrobacterium Fabrum str. 1D1416 for Citrus Transformation,” Microorganisms, vol. 12, no. 10, pp. 1-19, 1999.
    [CrossRef] [Google Scholar] [Publisher Link]
  2. Douglas Charles Blood, James Arnold Henderson, and Otto M. Radostits, Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs and Horses, 5th ed., 1981.
    [Google Scholar]
  3. Anis Ben Hsouna et al., “Citrus Lemon Essential Oil: Chemical Composition, Antioxidant and Antimicrobial Activities with its Preservative Effect against Listeria Monocytogenes Inoculated in Minced Beef Meat,” Lipids in Health and Disease, vol. 16, no. 1, pp. 1-11, 2017.
    [CrossRef] [Google Scholar] [Publisher Link]
  4. Wei Nee Cheng, and Sung Gu Han, “Bovine Mastitis: Risk Factors, Therapeutic Strategies, and Alternative Treatments-A Review,” Asian-Australasian journal of Animal Sciences, vol. 33, no. 11, pp. 1699-1713, 2020.
    [CrossRef] [Google Scholar] [Publisher Link]
  5. Huang ChunYan et al., “Evaluation of Antioxidant and Antitumour Activities of Lemon Essential Oil,” Journal of Medicinal Plants Research, vol. 4, no. 18, pp. 1910-1915, 2010.
    [
    Google Scholar] [Publisher Link]
  6. Norm G. Ducharme et al., “Invasive Teat Surgery in Dairy Cattle: Part I-Surgical Procedures and Classification of Lesions,” The Canadian Veterinary Journal, vol. 28, no. 12, pp. 757-762, 1987.
    [
    Google Scholar]
  7. Sumit Grover et al., “Physicochemical Assessment, Characterization, Antioxidant and Antimicrobial Potential of Essential Oil Extracted from Peel of Different Galgal (Citrus Pseudolimon) Cultivars,” Research Square, pp. 1-15, 2023.
    [CrossRef] [Google Scholar] [Publisher Link]
  8. S.S. Handa, “An Overview of Extraction Techniques for Medicinal and Aromatic Plants,” Extraction Technologies for Medicinal and Aromatic Plants,” vol. 1, no. 1, pp. 21-40, 2008.
    [Google Scholar]
  9. Seiichiro Ishihara, and Hisashi Haga, “Matrix Stiffness Contributes to Cancer Progression by Regulating Transcription Factors,” Cancers, vol. 14, no. 4, pp. 1-17, 2022.
    [CrossRef] [Google Scholar] [Publisher Link]
  10. G.M. Kamal et al., “Yield and Chemical Composition of Citrus Essential Oils as Affected by Drying Pretreatment of Peels,” International Food Research Journal, vol. 18, no. 4, pp. 1275-1282, 2011.
    [
    Google Scholar]
  11. Xingchi Kan et al., “Mammary Fibrosis Tendency and Mitochondrial Adaptability in Dairy Cows with Mastitis,” Microorganisms, vol. 12, no. 11, pp. 1-18, 2022.
    [CrossRef] [Google Scholar] [Publisher Link]
  12. Hardeep Kaur et al., “Citrus Pseudolimon: An Overview of Unexplored and Underutilised Plant from Genus Citrus,” Current Indian Science, vol. 3, no. 1, pp. 1-11, 2025.
    [CrossRef] [Google Scholar] [Publisher Link]
  13. Karthick Kumar, “Successful Treatment of Chronic Fibrosed Mastitis with Teat Fibrosis in Cows by Homeopathic Remedies: A Review of 18 Cases in Field Study,” International Journal of Current Microbiology and Applied Sciences, vol. 9, no. 8, pp. 3194-3197, 2020.
    [CrossRef] [Google Scholar] [Publisher Link]
  14. S.S. Makkar, “A Study of Chronic Teat Fibrosis in Bovines Treated with Homeopathy,” Allgemeine Homöopathische Zeitung, vol. 262, no. 2, pp. 2-76, 2017.
    [CrossRef] [Google Scholar] [Publisher Link]
  15. Ludovic Micallef et al., “The Myofibroblast, Multiple Origins for Major Roles in Normal and Pathological Tissue Repair,” Fibrogenesis and Tissue Repair, vol. 5, no. S1, pp. 1-5, 2012.
    [CrossRef] [Google Scholar] [Publisher Link]
  16. Muhammad Mohsin et al., “Chronic Mastitis: Leading Cause of Udder Fibrosis and Different Means of its Management,” Agrobiological Records, vol. 8, pp. 13-21, 2022.
    [CrossRef] [Google Scholar] [Publisher Link]
  17. H.R. Parsani et al., “Efficacy of a Homeopathic Complex (Masta-Forte+Actino Cure) and Antibiotics in Treatment of Bovine Clinical Mastitis,” International Journal of Bio-resource and Stress Management, vol. 14, no. 4, pp. 637-642, 2023.
    [CrossRef] [Google Scholar] [Publisher Link]
  18. Elizabeth F. Redente et al., “Loss of Fas Signaling in Fibroblasts Impairs Homeostatic Fibrosis Resolution and Promotes Persistent Pulmonary Fibrosis,” JCI Insight, vol. 6, no. 1, pp. 1-21, 2020.
    [CrossRef] [Google Scholar] [Publisher Link]
  19. Khurshid Ahmad Shah, Syed Anjum Andrabi, and Syed Sumbul, “A Study on Homeopathic Treatment of Teat Fibrosis in Bovines,” VetScan, vol. 5, no. 1, 2010.
    [Google Scholar] [Publisher Link]
  20. Ankita Thakur et al., “Innovative Applications of Hill Lemon (Citrus Pseudolimon Tanaka) in Sustainable Bioprocessing: Bioactive Extraction, Nanoparticle Synthesis, and Functional Food Products,” Journal of Food Biochemistry, vol. 2025, no. 1, pp. 1-15, 2025.
    [CrossRef] [Google Scholar] [Publisher Link]
  21. Nitika Thakur, and Swati Kumari, “Preliminary Screening of Phytochemicals and Antimicrobial Activity of Citrus Pseudolimon,” Advances in Traditional Medicine, vol. 22, no. 2, pp. 425-435, 2021.
    [CrossRef] [Google Scholar] [Publisher Link]
  22. Victor J. Thannickal et al., “Fibrosis: Ultimate and Proximate Causes,” The Journal of clinical Investigation, vol. 124, no. 11, pp. 4673-4677, 2014.
    [CrossRef] [Google Scholar] [Publisher Link]
  23. Jiang Zhang et al., “Mechanism of Astragalus Polysaccharide in Alleviating Bovine Mammary Fibrosis Through ROS/NLRP3 Inhibition and EMT Regulation,” Antioxidants, vol. 14, no. 5, pp. 1-16, 2025.
    [CrossRef] [Google Scholar] [Publisher Link]